Detailed information of TA system TA162054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 435279..435850
• Replicon: chromosome
• Accession: NZ_CP055231
• Organism: Enterococcus hirae strain 13144

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HU256_RS01995
• Protein ID: WP_135982259.1
• Coordinates: 435279..435620 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I6T4M0
• Locus tag: HU256_RS02000
• Protein ID: WP_010738535.1
• Coordinates: 435620..435850 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU256_RS01975	430502..431503	+	1002	WP_034866081.1	aspartate-semialdehyde dehydrogenase	-
HU256_RS01980	431500..432849	+	1350	WP_010720195.1	aspartate kinase	-
HU256_RS01985	432809..433918	+	1110	WP_025480561.1	amidohydrolase	-
HU256_RS01990	433948..435114	+	1167	WP_081123479.1	pyridoxal phosphate-dependent aminotransferase	-
HU256_RS01995	435279..435620	-	342	WP_135982259.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HU256_RS02000	435620..435850	-	231	WP_010738535.1	hypothetical protein	Antitoxin
HU256_RS02005	436310..437689	+	1380	WP_010738534.1	FAD-dependent oxidoreductase	-
HU256_RS02010	437870..438835	-	966	WP_034866085.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13089.40 Da
• Isoelectric Point: 10.1962

>T162054 WP_135982259.1 NZ_CP055231:c435620-435279 [Enterococcus hirae]
VPKARNYIPKKGDIVWIDFDPSTGKEIQKRRPGLVVSRYEFNCTTMFAIICPITSTIKNLPTRYSLPKDLDTKGQVLTSQ
LKSLDFKERKLKKVENLPLQDMAKIDQIIQYIF

Nucleotide

• Length: 342 bp

>T162054 NZ_CP071512:795751-795957 [Staphylococcus haemolyticus]
ATGAACTTTAGTGAAATTAGAATTAGTGAATTAAATACTGAAGAGCAAGCTAATTCGCTCCAACATAGACTTGAGAAGAT
GATAGGTGTAGATGAAGTAATTGTAGAACTAAATGACCAATTAGTTAAAATTTCTTATGAAACACCAACGAATTTAAATA
GTATTGAAAAAGAAATTTATGATGATGGTTATAAGGTATTAGGTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8577.57 Da
• Isoelectric Point: 4.1196

>AT162054 WP_010738535.1 NZ_CP055231:c435850-435620 [Enterococcus hirae]
MLITKSRLQGSSVVITLPSDNGKKPSENQEYIVVYSEDGTITLVPKIEDPFSAGQEAEYYEKDEWKDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT162054 NZ_CP071512:795446-795739 [Staphylococcus haemolyticus]
TTGAATAATCATGAAAATGCACATCATTCCAAACAAATTAAAATGAATTTAAAATCAAGACTAAATCGTATTGAAGGGCA
AGTTAGAGCGATTAATCGCATGATTGAAGAAGATGTATACTGTGATGACGTGCTAACTCAAATTAGAGCTACAAGATCTG
CATTAAATAGTGTGGCCACTAAGTTGCTAGATCATCATATGAAAAGCTGTATCATGGATAAAGTGAATAACGGCGCTCAA
GAAGAAGCGATGGAAGAATTGTTAGTGACATTTCAAAAATTAATGAAAGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A449E900