Detailed information of TA system TA162053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 184141..184712
• Replicon: chromosome
• Accession: NZ_CP055229
• Organism: Enterococcus hirae strain 13344

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HU262_RS00770
• Protein ID: WP_178309135.1
• Coordinates: 184141..184482 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I6T4M0
• Locus tag: HU262_RS00775
• Protein ID: WP_010738535.1
• Coordinates: 184482..184712 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU262_RS00750	179365..180366	+	1002	WP_178309134.1	aspartate-semialdehyde dehydrogenase	-
HU262_RS00755	180363..181712	+	1350	WP_081125345.1	aspartate kinase	-
HU262_RS00760	181672..182781	+	1110	WP_164907387.1	amidohydrolase	-
HU262_RS00765	182811..183977	+	1167	WP_081123479.1	pyridoxal phosphate-dependent aminotransferase	-
HU262_RS00770	184141..184482	-	342	WP_178309135.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HU262_RS00775	184482..184712	-	231	WP_010738535.1	hypothetical protein	Antitoxin
HU262_RS00780	185172..186551	+	1380	WP_010738534.1	FAD-dependent oxidoreductase	-
HU262_RS00785	186732..187697	-	966	WP_034866085.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13100.47 Da
• Isoelectric Point: 10.3215

>T162053 WP_178309135.1 NZ_CP055229:c184482-184141 [Enterococcus hirae]
VPKARNYIPKKGDIVWIDFNPSTGKEIQKRRPGLVVSRYEFNCTTMFAIICPITSTIKNLPTRYSLPKDLDTKGQVLISQ
LKSLDFKERKLKKVENLPLQDMAKIDQIIQYIF

Nucleotide

• Length: 342 bp

>T162053 NZ_CP071508:958291-958440 [Staphylococcus haemolyticus]
ATGACTAAAAATAAAGATTCAGAATTAAATTATCATGAGGAAGAAAATGCGATGGTTCAAGATTTAGATGACTTGAAAAC
ATTGGGCAAAGAAATGGAACAAATTTCTGAAGAAAACGACGAGGACAGGTTGAATCAATCGCATGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8577.57 Da
• Isoelectric Point: 4.1196

>AT162053 WP_010738535.1 NZ_CP055229:c184712-184482 [Enterococcus hirae]
MLITKSRLQGSSVVITLPSDNGKKPSENQEYIVVYSEDGTITLVPKIEDPFSAGQEAEYYEKDEWKDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT162053 NZ_CP071508:957669-958268 [Staphylococcus haemolyticus]
ATGGCAATGAACTTTAAAGTATTTAATGATGTAGAACATGTAGCTGAGTACACAGCAGATATCATTAGAAAGCAATTTAA
TAACAACCCTACAACTATTGCGGGAATTCATTTAACAAAGGATGCTGCACCTGTTTTAGATGAGTTAAAGAAAGATGTAG
ATCATAATGCGGTTGATTTTAGTCAAGTAAATATCTTAGATTATGACGATAATCGCTCATACTATGAAGCATTAGGTGTA
CCTACTAGTCAAATTTATCCGATTAATCTTGACGATGATGCAGAATCTTTAATTGATGACAAAATTAAAACTAAAGAAAA
TAAAGGTAAATTAATTTTACAAGTTACTTCTATTGATGAATCAGGTAGTTTAAATGTAAATGTAAGACAAGGATTATTAA
AAGCGCGTGAAGTAGTATTAGTTGTGACTGGTGCTAATAAACGTGAAGTTGTTAAAAAACTTTATGAAGAAAATGGTAAA
TCTAGCTTTGAACCAGCTGATTTAAAAGCTCATAGAATGGTAACAGTTGTTTTAGATAGAGCAGCTGCAGAAGGTTTACC
TGAAGATGTTAAAGAATATTTCACTGCTAGATTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A449E900