Detailed information of TA system TA162049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2866844..2867297
• Replicon: chromosome
• Accession: NZ_CP055227
• Organism: Erwinia amylovora strain Ea1189

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GTU81_RS13160
• Protein ID: WP_013035888.1
• Coordinates: 2866844..2867080 (-)
• Length: 79 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D4HZA2
• Locus tag: GTU81_RS13165
• Protein ID: WP_004159527.1
• Coordinates: 2867070..2867297 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTU81_RS13140	2862359..2863237	-	879	WP_004159509.1	Fe(3+)-hydroxamate ABC transporter substrate-binding protein FhuD	-
GTU81_RS13145	2863252..2864046	-	795	WP_004159512.1	Fe3+-hydroxamate ABC transporter ATP-binding protein FhuC	-
GTU81_RS13150	2864095..2866311	-	2217	Protein_2552	ferrichrome porin FhuA	-
GTU81_RS13155	2866639..2866857	+	219	WP_004159522.1	hypothetical protein	-
GTU81_RS13160	2866844..2867080	-	237	WP_013035888.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTU81_RS13165	2867070..2867297	-	228	WP_004159527.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GTU81_RS13170	2867618..2867905	+	288	WP_004159528.1	SymE family type I addiction module toxin	-
GTU81_RS13175	2867969..2868472	-	504	WP_048901601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-78)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-75)

Sequences

Toxin

Protein

• Length: 79 a.a.
• Molecular weight: 9135.79 Da
• Isoelectric Point: 9.0876

>T162049 WP_013035888.1 NZ_CP055227:c2867080-2866844 [Erwinia amylovora]
ISYELLFDPWALKEWKKLGATVREQFKKKLAEVLVNLQVESARLRDHPDCCKIKLKSSGYRLVYQVQDEQLVMLVISV

Nucleotide

• Length: 237 bp

>T162049 NZ_CP071505:806616-806981 [Staphylococcus haemolyticus]
ATGATTAGAAGAGGCGATGTATATTTAGCTGATTTGTCACCAGTACAGGGCTCAGAACAAGGGGGAGTCAGACCTGTAGT
TATCATTCAAAACGATACTGGCAACAAATATAGTCCTACCGTAATCGTTGCTGCGATTACGGGAAGGATTAATAAAGCGA
AAATACCCACACATGTTGAAATCGAGAAAAAGAAATACAGACTTGATAAAGATTCAGTAATTCTACTCGAGCAAATTAGA
ACATTAGATAAAAAACGACTAAAAGAAAAATTAACATATCTATCTGATGAGAAAATGCAAGAAGTAGATGAAGCACTAGA
CATTAGTCTAGGACTGCATGATGAAGTTAAAACGCAAAACACATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8435.90 Da
• Isoelectric Point: 7.9766

>AT162049 WP_004159527.1 NZ_CP055227:c2867297-2867070 [Erwinia amylovora]
VRLDDKLKSKACAVLDELDVSPTEAVRLLFQYIAENERLPLKALTVSDEKDALLKTVRERLASLQKGIKVTLDKL

Nucleotide

• Length: 228 bp

>AT162049 NZ_CP071505:806449-806619 [Staphylococcus haemolyticus]
ATGCTTACTTTCAATCAAAATAGAAGTCACAGTCTTGAACAATCTTTAAAAGAAGGCTATTCACAAATGGCTGAATTAAA
TCTCTCCCTAGCGACAGAAGCTTTTCCTCTAGAATGCGAAGCTTGTGATTGCAACGAAACGTACATTATTTCTAATTATA
AAAGTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A831A410