Detailed information of TA system TA162046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1083644..1084271
• Replicon: chromosome
• Accession: NZ_CP055227
• Organism: Erwinia amylovora strain Ea1189

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: GTU81_RS05010
• Protein ID: WP_004156337.1
• Coordinates: 1083644..1083862 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: GTU81_RS05015
• Protein ID: WP_013035913.1
• Coordinates: 1083882..1084271 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTU81_RS04980	1079630..1079968	+	339	WP_004156319.1	P-II family nitrogen regulator	-
GTU81_RS04985	1079986..1081287	+	1302	WP_004172214.1	ammonium transporter AmtB	-
GTU81_RS04990	1081362..1082222	-	861	WP_004156324.1	acyl-CoA thioesterase II	-
GTU81_RS04995	1082447..1083004	+	558	WP_004156333.1	YbaY family lipoprotein	-
GTU81_RS05000	1083027..1083344	-	318	WP_004156334.1	MGMT family protein	-
GTU81_RS05010	1083644..1083862	-	219	WP_004156337.1	hemolysin expression modulator Hha	Toxin
GTU81_RS05015	1083882..1084271	-	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
GTU81_RS05020	1084418..1084771	-	354	WP_004156342.1	hypothetical protein	-
GTU81_RS05025	1085543..1085689	-	147	WP_004156349.1	type B 50S ribosomal protein L36	-
GTU81_RS05030	1085677..1085958	-	282	WP_004156350.1	type B 50S ribosomal protein L31	-
GTU81_RS05035	1086106..1089252	-	3147	WP_004156353.1	multidrug efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T162046 WP_004156337.1 NZ_CP055227:c1083862-1083644 [Erwinia amylovora]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T162046 NZ_CP071503:2726662-2726943 [Shewanella sp. FJAT-51800]
ATGACCTATAAGCTCAAATTTGATAAAAGAGCTTTGAAAGAATGGGAAAAATTAGGCTATCCGGTAAAAACTCAATTCAA
AAAGAAATTAGAAGAACGCCTTGAGAATCCCCGAGTACCAAGCGCCAAATTAAGTGGTCATGCAGACCGCTACAAAATAA
AATTACGTTCAGCTGGTTATCGGTTGGTATATCAGGTTTTTGATGAAGAAATCGTTGTTACCGTGATTGCGATTGGCAAG
CGGGAAAACTCAGATGTTTACTCGTTGGCAGACAATCGCTGA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT162046 WP_013035913.1 NZ_CP055227:c1084271-1083882 [Erwinia amylovora]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT162046 NZ_CP071503:2726421-2726672 [Shewanella sp. FJAT-51800]
ATGGCGTATCAAATTTTGACATCTGTCGCAGCAAGTATCACCGAGCTAAAAAAGAACCCTATGGGTACGGCCTTGGAAGG
TGAAGGACAGCCTGTGGCAATTCTCAATAGAAACGAACCTGCGTTTTATATCGTACCTCCGGCTCTGTTTGGCTACTTTC
AAGAATTAGCTGAAGATGCAGAATTGAACCGTATCGCAGATGAGCGGATGCAAGAGCCAGATTTTGTCGAGGTCAACATC
GATGACCTATAA

Structures

Toxin

• AlphaFold DB: A0A831EPQ0