Detailed information of TA system TA162035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2189895..2190424
• Replicon: chromosome
• Accession: NZ_CP055225
• Organism: Staphylococcus aureus LAC

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HUW68_RS10955
• Protein ID: WP_000621175.1
• Coordinates: 2189895..2190257 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HUW68_RS10960
• Protein ID: WP_000948331.1
• Coordinates: 2190254..2190424 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW68_RS10935	2186873..2187643	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
HUW68_RS10940	2187618..2188097	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
HUW68_RS10945	2188099..2188425	-	327	WP_001052491.1	anti-sigma factor antagonist	-
HUW68_RS10950	2188544..2189545	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HUW68_RS10955	2189895..2190257	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUW68_RS10960	2190254..2190424	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HUW68_RS10965	2190509..2191657	-	1149	WP_001281145.1	alanine racemase	-
HUW68_RS10970	2191723..2192082	-	360	WP_000581200.1	holo-ACP synthase	-
HUW68_RS10975	2192086..2192577	-	492	WP_001205910.1	PH domain-containing protein	-
HUW68_RS10980	2192564..2194147	-	1584	WP_001294626.1	PH domain-containing protein	-
HUW68_RS10985	2194140..2194619	-	480	WP_001287088.1	hypothetical protein	-
HUW68_RS10990	2194827..2195387	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T162035 WP_000621175.1 NZ_CP055225:c2190257-2189895 [Staphylococcus aureus LAC]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T162035 NZ_CP071491:c384539-384204 [Glaesserella parasuis]
ATGTCAGGAATTATCAACCAAATCGTCATTACAGATAATTTTTCAAAGTGGCTAGATGGTTTAAAAAATCCTATTGCTAA
AGCAACAGTGGCAAGAAGAATTAAGAATACAGTAAAAGGTAATTTTGATGATCATAAAGCGCTTGCAGGTACTGGTGGAC
TTTATGAAATGCGAATAGCGACAGGCGCTGGTTATCGAATTTACTATGCACAGCAGGGCGATGTTATTTATATTCTGTTA
CAAGGTAGTGATAAATCAACACAGCAAGTCGATATTGAGAAAGCGAAAGCATTGTGGGCAGAATTAAAACAGACAGGAGG
AAGTAATGAATATTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT162035 WP_000948331.1 NZ_CP055225:c2190424-2190254 [Staphylococcus aureus LAC]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT162035 NZ_CP071491:c384196-383900 [Glaesserella parasuis]
ATGGCAGAAAAACACGGTTTACGTCCTTTTGATGTCGCTGAATTTTTGGATAACGAAGAAACAATACAAGCCTATTTAGC
AGAAGTATTAAAAGAGGGCGATCAAGATGAGTTTTTGGAAGCCCTTAATGATGTAGCAAGAGCAAGGGGAATCGCAGAAC
TTGCGAAGCAAACGGGGTTAGGTAGAGAAAGTTTATATAAAACCCTATCTAAAGGAAGTAAACCTCGTTTTGAAACTATT
CAAAAAATTTTAGGAGCATTTAATCTTGTGCTTATCCCACAAATAAAAAATAGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1