Detailed information of TA system TA162021

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4084089..4084749
• Replicon: chromosome
• Accession: NZ_CP055224
• Organism: Pectobacterium atrosepticum strain Green1

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: Q6D959
• Locus tag: HLB43_RS18005
• Protein ID: WP_011092367.1
• Coordinates: 4084089..4084502 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: Q6D960
• Locus tag: HLB43_RS18010
• Protein ID: WP_011092366.1
• Coordinates: 4084483..4084749 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLB43_RS17980 (HLB43_17975)	4079233..4079847	-	615	WP_039296937.1	recombinase family protein	-
HLB43_RS17985 (HLB43_17980)	4080367..4081485	+	1119	WP_215216026.1	hypothetical protein	-
HLB43_RS17990 (HLB43_17985)	4082001..4082519	-	519	WP_215216027.1	lysozyme inhibitor LprI family protein	-
HLB43_RS17995 (HLB43_17990)	4082516..4082710	-	195	WP_215216028.1	hypothetical protein	-
HLB43_RS18000 (HLB43_17995)	4083025..4083405	-	381	WP_215216029.1	hypothetical protein	-
HLB43_RS18005 (HLB43_18000)	4084089..4084502	-	414	WP_011092367.1	protein YgfX	Toxin
HLB43_RS18010 (HLB43_18005)	4084483..4084749	-	267	WP_011092366.1	FAD assembly factor SdhE	Antitoxin
HLB43_RS18015 (HLB43_18010)	4084985..4085986	+	1002	WP_011092365.1	tRNA-modifying protein YgfZ	-
HLB43_RS18020 (HLB43_18015)	4086210..4086857	-	648	WP_039296921.1	hemolysin III family protein	-
HLB43_RS18025 (HLB43_18020)	4087276..4087884	+	609	WP_011092363.1	HD domain-containing protein	-
HLB43_RS18030 (HLB43_18025)	4087938..4088942	+	1005	WP_039296919.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16397.31 Da
• Isoelectric Point: 10.3509

>T162021 WP_011092367.1 NZ_CP055224:c4084502-4084089 [Pectobacterium atrosepticum]
VAQWQCDLRVSWRMQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQ
EWQIVKRPWLLKNGVLLALQSINDKERQQLWLASDSMGDDEWRHLRQLLLQQKHWAR

Nucleotide

• Length: 414 bp

>T162021 NZ_CP071489:972132-972476 [Glaesserella parasuis]
ATGGAAACAGAAGAATATCTTACTTTTATTGAAACCAAAGTTTTTGAAGAAGACCGAAAAGCATTAATGTCTGATGATGA
ATATCAGAAATTTCAGGCTTATTTATTAGAATCTCACGAATTAGGCGATTTTATTCAGAATACTGGTGGTTGCCAAAAAA
TTCGCTGGAAACTAGAAAGTAATAACAAGGGCAAAAGTGGTGGTGTAAGGGTGATTTACTATGTTGTAACCAAACAGGGA
AAATTATTATTAATGATGATGTATGCTAAAAGCAAACAAGACAATATGAGTGATAAACAAAAAGCAATGTTAAAAGCGGT
TGTATCCCAATTAAGTGAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10462.94 Da
• Isoelectric Point: 6.2223

>AT162021 WP_011092366.1 NZ_CP055224:c4084749-4084483 [Pectobacterium atrosepticum]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDSDKRAFVRLLQSDDPDLFNWLMNHGEPKDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT162021 NZ_CP071489:972479-972775 [Glaesserella parasuis]
ATGGAAAAAGAACTTTTTGAACGTCTAATGGAAAGTGCGAGAGAAATGGTCGCCATTGAAAAAGGCGAAATAGAAGTACC
ACCGCATCGTATTCATATCCATAAAATCCCGAATGTCAAAGCGATTAGAACCCAGCACCAGATGAAACAAGCGGAATTTG
CTTCCCTTTTAGGCGTAAGCCAAAATCTTGTGCAGTCGTGGGAAATCGGCAGACGAACCCCAAATGGCGCTGCACGGAAA
TTGCTTGCGATTATTGAGCAACAGCCTAATCTTGTTCCAGTACTTCAGAATTTGTAG

Structures

Toxin

• AlphaFold DB: Q6D959

Antitoxin

• AlphaFold DB: Q6D960