Detailed information of TA system TA162018

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 2939814..2940538
• Replicon: chromosome
• Accession: NZ_CP055224
• Organism: Pectobacterium atrosepticum strain Green1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HLB43_RS13165
• Protein ID: WP_039291161.1
• Coordinates: 2939814..2940134 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HLB43_RS13170
• Protein ID: WP_039291159.1
• Coordinates: 2940203..2940538 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLB43_RS13140 (HLB43_13135)	2935326..2935544	+	219	WP_011094380.1	KTSC domain-containing protein	-
HLB43_RS13145 (HLB43_13140)	2935888..2936835	-	948	WP_039291164.1	LysR family transcriptional regulator	-
HLB43_RS13150 (HLB43_13145)	2936947..2937975	+	1029	WP_011094382.1	subclass B3 metallo-beta-lactamase CAR-1	-
HLB43_RS13155 (HLB43_13150)	2938534..2938731	-	198	WP_051122287.1	hypothetical protein	-
HLB43_RS13160 (HLB43_13155)	2938863..2939696	-	834	WP_039294889.1	DUF4942 domain-containing protein	-
HLB43_RS13165 (HLB43_13160)	2939814..2940134	-	321	WP_039291161.1	TA system toxin CbtA family protein	Toxin
HLB43_RS13170 (HLB43_13165)	2940203..2940538	-	336	WP_039291159.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HLB43_RS13175 (HLB43_13170)	2940578..2941051	-	474	WP_039291157.1	DNA repair protein RadC	-
HLB43_RS13180 (HLB43_13175)	2941174..2941524	-	351	WP_039291155.1	hypothetical protein	-
HLB43_RS13185 (HLB43_13180)	2941582..2942415	-	834	WP_039291153.1	hypothetical protein	-
HLB43_RS13190 (HLB43_13185)	2942562..2942720	-	159	WP_155242703.1	hypothetical protein	-
HLB43_RS13195 (HLB43_13190)	2942904..2943791	-	888	WP_039291149.1	50S ribosome-binding GTPase	-
HLB43_RS13200 (HLB43_13195)	2943886..2944812	-	927	WP_039291148.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12022.73 Da
• Isoelectric Point: 4.7627

>T162018 WP_039291161.1 NZ_CP055224:c2940134-2939814 [Pectobacterium atrosepticum]
MQTSPAIPPREDNPCPSPITVWQQLLTYLLEKHYGLTLNDTPFCEENVIQAHIDAGVTLVNAVNFLVEKYELVRIDRDGF
NWQEQSPFLTAVDVLRARRATGLLKA

Nucleotide

• Length: 321 bp

>T162018 NZ_CP071489:454476-454652 [Glaesserella parasuis]
ATGAAACAAAGTGAATTTTTAAGATGGCTAATTGCTCAAGGGGTAGTAAAGGAAGAAGGCTCAAAGCACATCAAACTTTA
TTACAACGGTAATCAATCAACAATGCCAAGACAACCTAGCAAGGAAATCAAAAACGGCACAATGAGAGCGATTAAAAAGC
AATTAGGTTTAAACTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12468.28 Da
• Isoelectric Point: 6.6874

>AT162018 WP_039291159.1 NZ_CP055224:c2940538-2940203 [Pectobacterium atrosepticum]
MPSSNTPEWGLKCAVTPRFGARLVQEGHRLHYLADRASIVGTFSKTEAQNLERCFPELIKLLEQKLRMGELNPRQQNCVT
LHCNEWICEADTLGSVGYVYIVIYPSSAAAE

Nucleotide

• Length: 336 bp

>AT162018 NZ_CP071489:454699-455112 [Glaesserella parasuis]
ATGCTGTATCCAGCACGATTCGAATTTGAAGATGGTAGTTATAATGTTTCATTCCGTGATATTCCTGAAGCGTTGACTTG
CGGTGATAACTACGAAGACGCTTTAGAGATGGCAAAAGATGCATTGCTTACCAGTATGGATTTTTATTTCGAAGATCATC
GCAAAGTACCGTTACCAAGCCCACCACAAGAAGGCGAAGTGCTGATCGAATTACCGACAAGTATTTTCGCCAAAGTGCTA
TTGCTAAATGAGATGGTCGATCAGAATATCTCCAATGTGGAATTAGCAAAACGAATTGCCGTAAAACCGCAAGAAGTACA
GCGTATTACTAATCTTGGGCATAGTACAAAAATCGATACTATTGCAAGAGCGATTAATGCACTCGACAAACAGTTTGAAT
TACGTGTTGTCTAA