Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4075639..4076257 Replicon chromosome
Accession NZ_CP055219
Organism Shigella flexneri strain STEFF_12

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ41_RS19955 Protein ID WP_001291435.1
Coordinates 4075639..4075857 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ41_RS19960 Protein ID WP_000344800.1
Coordinates 4075883..4076257 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ41_RS19925 (4070800) 4070800..4071432 + 633 WP_152917029.1 recombinase family protein -
HUZ41_RS19930 (4071650) 4071650..4071877 + 228 WP_024183738.1 DUF2188 domain-containing protein -
HUZ41_RS19935 (4072221) 4072221..4072574 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HUZ41_RS19940 (4072616) 4072616..4074166 - 1551 WP_024238838.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ41_RS19945 (4074330) 4074330..4074800 - 471 WP_000136192.1 YlaC family protein -
HUZ41_RS19950 (4074916) 4074916..4075467 - 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ41_RS19955 (4075639) 4075639..4075857 - 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ41_RS19960 (4075883) 4075883..4076257 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ41_RS19965 (4076803) 4076803..4079952 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ41_RS19970 (4079975) 4079975..4081168 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T162011 WP_001291435.1 NZ_CP055219:c4075857-4075639 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T162011 NZ_CP071464:c4902681-4902397 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT162011 WP_000344800.1 NZ_CP055219:c4076257-4075883 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT162011 NZ_CP071464:c4902913-4902671 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References