Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1860503..1861086 Replicon chromosome
Accession NZ_CP055219
Organism Shigella flexneri strain STEFF_12

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag HUZ41_RS09325 Protein ID WP_000254738.1
Coordinates 1860503..1860838 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1V2T5M9
Locus tag HUZ41_RS09330 Protein ID WP_000581941.1
Coordinates 1860838..1861086 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ41_RS09305 (1855563) 1855563..1856330 + 768 Protein_1832 YgcG family protein -
HUZ41_RS09310 (1856390) 1856390..1857688 - 1299 WP_000036723.1 phosphopyruvate hydratase -
HUZ41_RS09315 (1857776) 1857776..1859413 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HUZ41_RS09320 (1859641) 1859641..1860432 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HUZ41_RS09325 (1860503) 1860503..1860838 - 336 WP_000254738.1 endoribonuclease MazF Toxin
HUZ41_RS09330 (1860838) 1860838..1861086 - 249 WP_000581941.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HUZ41_RS09335 (1861164) 1861164..1863398 - 2235 WP_000226815.1 GTP pyrophosphokinase -
HUZ41_RS09340 (1863446) 1863446..1864747 - 1302 WP_023281448.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T162002 WP_000254738.1 NZ_CP055219:c1860838-1860503 [Shigella flexneri]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T162002 NZ_CP071458:132460-132879 [Rhizobium lentis]
ATGGCGCGCTACATGCTCGACACGAACATGTGTATCTACCTGATGAAAAACCAGCCGGAGCAGGTCGCTCGCCGCTTTGC
CAGTTGCTATGTGGGGGACGTGGTGATGTCCGCCATCACCTTCGCGGAACTCGAGTACGGTGTTTCCGCATCCGATGATC
CGGAACGAGAACTTGATAATCTCACCGCCCTGGCCGAGCTAATCACGGTAGAGCCGTTTGGGATCGCAGCAGCGCGTGCC
TACGGTCCGGTTCGAATGGCGACGAGAGACCGGAAGAAAGATCATCTCGACAAACTGATCGCATCGCATGCGATCGCTCT
GGACACGGTATTGGTGACCAACAACACGCGGGACTTCCTGGCGTATCCCGGACTGAAGCTCGAAAACTGGCTGGACGCGG
AACGCAGAGAAACCAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9385.76 Da        Isoelectric Point: 4.4665

>AT162002 WP_000581941.1 NZ_CP055219:c1861086-1860838 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT162002 NZ_CP071458:132224-132460 [Rhizobium lentis]
ATGCACACCACTAAAGCTTTCAAGAATGGAAATTCCCAAGCGGTTCGCATACCGGCGGAACTGGCGTTTGAGCGAACCGA
TGTTGAGTTGGAGATCGAGCGCATCGGCGACGAGCTTCGCATCCGTCCTGTGCATCGCTCCCTGGCCGGCGTTCTGGATG
TATTCGCCCGCTTCTCTCCGGATTTCATGCGTGACGGAAGAGAGTCCCATGAGCAAGACGAGCGGGACAAGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2T5M9

References