Detailed information of TA system TA161991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 356923..357561
• Replicon: chromosome
• Accession: NZ_CP055219
• Organism: Shigella flexneri strain STEFF_12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HUZ41_RS01880
• Protein ID: WP_000813794.1
• Coordinates: 356923..357099 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUZ41_RS01885
• Protein ID: WP_001270286.1
• Coordinates: 357145..357561 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ41_RS01860 (352542)	352542..353717	-	1176	WP_001236265.1	BenE family transporter YdcO	-
HUZ41_RS01865 (353809)	353809..354345	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HUZ41_RS01870 (354418)	354418..356379	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HUZ41_RS01875 (356471)	356471..356701	-	231	WP_000494244.1	YncJ family protein	-
HUZ41_RS01880 (356923)	356923..357099	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HUZ41_RS01885 (357145)	357145..357561	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HUZ41_RS01890 (357640)	357640..359046	+	1407	WP_239637038.1	PLP-dependent aminotransferase family protein	-
HUZ41_RS01895 (359291)	359291..360436	+	1146	WP_000047418.1	ABC transporter substrate-binding protein	-
HUZ41_RS01900 (360454)	360454..361467	+	1014	WP_024238793.1	ABC transporter ATP-binding protein	-
HUZ41_RS01905 (361468)	361468..362409	+	942	WP_001251319.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vgrG/tssI / rhs/PAAR	341785..399016	57231
	-	flank	IS/Tn	-	-	351354..352502	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T161991 WP_000813794.1 NZ_CP055219:356923-357099 [Shigella flexneri]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T161991 NZ_CP071442:c51248-50967 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT161991 WP_001270286.1 NZ_CP055219:357145-357561 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT161991 NZ_CP071442:c51514-51245 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• PDB: 6HPB