Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 36064..36707 Replicon plasmid unnamed2
Accession NZ_CP055218
Organism Raoultella ornithinolytica strain STEFF_15

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag HUZ43_RS27755 Protein ID WP_000754566.1
Coordinates 36291..36707 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HUZ43_RS27750 Protein ID WP_001261276.1
Coordinates 36064..36294 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ43_RS27720 (HUZ43_27600) 31800..31904 + 105 WP_032409716.1 hypothetical protein -
HUZ43_RS27725 (HUZ43_27605) 32033..32290 + 258 WP_000764642.1 hypothetical protein -
HUZ43_RS27730 (HUZ43_27610) 32348..33124 - 777 WP_000015958.1 site-specific integrase -
HUZ43_RS27735 (HUZ43_27615) 33121..33864 - 744 WP_000129823.1 hypothetical protein -
HUZ43_RS27740 (HUZ43_27620) 33915..34265 - 351 WP_000493378.1 hypothetical protein -
HUZ43_RS27745 (HUZ43_27625) 34483..35603 + 1121 WP_085950818.1 IS3-like element ISSen4 family transposase -
HUZ43_RS27750 (HUZ43_27630) 36064..36294 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ43_RS27755 (HUZ43_27635) 36291..36707 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ43_RS27760 (HUZ43_27640) 36911..37767 + 857 Protein_41 IS3-like element ISEc15 family transposase -
HUZ43_RS27765 (HUZ43_27645) 38049..38249 + 201 Protein_42 transposase -
HUZ43_RS27770 (HUZ43_27650) 38272..38694 + 423 WP_032446862.1 hypothetical protein -
HUZ43_RS27775 (HUZ43_27655) 38703..39200 + 498 WP_032446861.1 hypothetical protein -
HUZ43_RS27780 (HUZ43_27660) 39337..39672 - 336 Protein_45 Tn3 family transposase -
HUZ43_RS27785 (HUZ43_27665) 40192..40275 + 84 Protein_46 SOS response-associated peptidase -
HUZ43_RS27790 40333..40404 - 72 Protein_47 hypothetical protein -
HUZ43_RS27795 (HUZ43_27670) 40438..41367 - 930 Protein_48 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / mph(A) / sul1 / qacE / catA2 / tet(A) - 1..48095 48095
- inside IScluster/Tn catA2 / tet(A) - 34483..46477 11994


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T161986 WP_000754566.1 NZ_CP055218:36291-36707 [Raoultella ornithinolytica]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T161986 NZ_CP071441:3686234-3686521 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT161986 WP_001261276.1 NZ_CP055218:36064-36294 [Raoultella ornithinolytica]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT161986 NZ_CP071441:3686508-3686810 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References