Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4393486..4394105 Replicon chromosome
Accession NZ_CP055216
Organism Raoultella ornithinolytica strain STEFF_15

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag HUZ43_RS21045 Protein ID WP_004858783.1
Coordinates 4393887..4394105 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag HUZ43_RS21040 Protein ID WP_004858785.1
Coordinates 4393486..4393860 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ43_RS21030 (HUZ43_20930) 4388596..4389789 + 1194 WP_004858789.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ43_RS21035 (HUZ43_20935) 4389812..4392958 + 3147 WP_004858787.1 multidrug efflux RND transporter permease subunit AcrB -
HUZ43_RS21040 (HUZ43_20940) 4393486..4393860 + 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
HUZ43_RS21045 (HUZ43_20945) 4393887..4394105 + 219 WP_004858783.1 HHA domain-containing protein Toxin
HUZ43_RS21050 (HUZ43_20950) 4394257..4394823 + 567 WP_004858781.1 maltose O-acetyltransferase -
HUZ43_RS21055 4394795..4394929 - 135 WP_223306663.1 hypothetical protein -
HUZ43_RS21060 (HUZ43_20955) 4394923..4395393 + 471 WP_004858780.1 YlaC family protein -
HUZ43_RS21065 (HUZ43_20960) 4395368..4396819 - 1452 WP_032716766.1 PLP-dependent aminotransferase family protein -
HUZ43_RS21070 (HUZ43_20965) 4396923..4397633 + 711 WP_004858778.1 GNAT family protein -
HUZ43_RS21075 (HUZ43_20970) 4397630..4397770 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
HUZ43_RS21080 (HUZ43_20975) 4397773..4398033 - 261 WP_004858777.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T161984 WP_004858783.1 NZ_CP055216:4393887-4394105 [Raoultella ornithinolytica]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T161984 NZ_CP071441:3613869-3614246 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT161984 WP_004858785.1 NZ_CP055216:4393486-4393860 [Raoultella ornithinolytica]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT161984 NZ_CP071441:3613411-3613779 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References