Detailed information of TA system TA161982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2826933..2827684
• Replicon: chromosome
• Accession: NZ_CP055216
• Organism: Raoultella ornithinolytica strain STEFF_15

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: HUZ43_RS13440
• Protein ID: WP_097357840.1
• Coordinates: 2827202..2827684 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A808Y4Y8
• Locus tag: HUZ43_RS13435
• Protein ID: WP_015584326.1
• Coordinates: 2826933..2827211 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ43_RS13420 (HUZ43_13380)	2822119..2823495	+	1377	Protein_2635	KUP/HAK/KT family potassium transporter	-
HUZ43_RS13425 (HUZ43_13385)	2823660..2824208	+	549	WP_228399960.1	DUF4451 domain-containing protein	-
HUZ43_RS13430 (HUZ43_13390)	2824366..2826291	-	1926	WP_239614510.1	C1 family peptidase	-
HUZ43_RS13435 (HUZ43_13395)	2826933..2827211	+	279	WP_015584326.1	DUF1778 domain-containing protein	Antitoxin
HUZ43_RS13440 (HUZ43_13400)	2827202..2827684	+	483	WP_097357840.1	GNAT family N-acetyltransferase	Toxin
HUZ43_RS13445	2827799..2827948	-	150	Protein_2640	ISNCY family transposase	-
HUZ43_RS13450 (HUZ43_13405)	2828128..2828286	-	159	WP_004861965.1	hypothetical protein	-
HUZ43_RS13455 (HUZ43_13410)	2829005..2829880	-	876	WP_100663434.1	NAD(P)-dependent oxidoreductase	-
HUZ43_RS13460 (HUZ43_13415)	2830142..2830858	-	717	WP_004861956.1	ABC transporter ATP-binding protein	-
HUZ43_RS13465 (HUZ43_13420)	2830855..2831730	-	876	WP_015584331.1	ATP-binding cassette domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17642.51 Da
• Isoelectric Point: 9.7381

>T161982 WP_097357840.1 NZ_CP055216:2827202-2827684 [Raoultella ornithinolytica]
MGMRPPEPLSTGHNIAEFCCQDQVLNEWLKKKALKNHSTGISRVFVVCAENTKRVIAYYSLSSGSVHRNTVPGAYRRNAP
EAVPVIVLGRLAVDAAWAGKGLGAALLKDAIYRTEHIAVQVGVRALLVHALNDEVREFYTRFGFEPSIINTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T161982 NZ_CP071441:3417752-3417925 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10714.30 Da
• Isoelectric Point: 4.6722

>AT161982 WP_015584326.1 NZ_CP055216:2826933-2827211 [Raoultella ornithinolytica]
MKPDVREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFASFMEELDAPMTAERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT161982 NZ_CP071441:3417428-3417724 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A808Y4Y8