Detailed information of TA system TA161981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 2496238..2497151
• Replicon: chromosome
• Accession: NZ_CP055216
• Organism: Raoultella ornithinolytica strain STEFF_15

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HUZ43_RS11685
• Protein ID: WP_220668027.1
• Coordinates: 2496238..2496711 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A808Y7A4
• Locus tag: HUZ43_RS11690
• Protein ID: WP_004862777.1
• Coordinates: 2496708..2497151 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ43_RS11660 (HUZ43_11645)	2492277..2492885	-	609	WP_032724246.1	glutathione S-transferase family protein	-
HUZ43_RS11665 (HUZ43_11650)	2493127..2494032	+	906	WP_032716408.1	transcriptional regulator GcvA	-
HUZ43_RS11670 (HUZ43_11655)	2494060..2494965	-	906	WP_004862787.1	LysR family transcriptional regulator	-
HUZ43_RS11675 (HUZ43_11660)	2495068..2495385	+	318	WP_004862785.1	nuclear transport factor 2 family protein	-
HUZ43_RS11680 (HUZ43_11665)	2495378..2496157	+	780	WP_004862782.1	SDR family oxidoreductase	-
HUZ43_RS11685 (HUZ43_11670)	2496238..2496711	-	474	WP_220668027.1	RES domain-containing protein	Toxin
HUZ43_RS11690 (HUZ43_11675)	2496708..2497151	-	444	WP_004862777.1	DUF2384 domain-containing protein	Antitoxin
HUZ43_RS11695 (HUZ43_11680)	2497341..2497907	-	567	WP_015584190.1	DJ-1/PfpI family protein	-
HUZ43_RS11700 (HUZ43_11685)	2498305..2499087	+	783	WP_032717546.1	DUF3750 domain-containing protein	-
HUZ43_RS11705 (HUZ43_11690)	2499284..2500507	+	1224	WP_004862767.1	zinc metallochaperone GTPase ZigA	-
HUZ43_RS11725 (HUZ43_11710)	2501442..2501861	-	420	WP_046852391.1	acid resistance repetitive basic protein Asr	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (15-147)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17553.86 Da
• Isoelectric Point: 5.0735

>T161981 WP_220668027.1 NZ_CP055216:c2496711-2496238 [Raoultella ornithinolytica]
MIFYRLVTSRYASEAWTGSGANQYGGRWNHQGHPAVYVSTSIALASLEILVHVKNEAILNQYQLYSIEIPNSEVESLDKQ
WLPDNWQENPMPVSTMDLGTGWLQSGSGLALMLPSCIIPYENNAILNPLHPAFSEALASVRQYPFTFDPRLARSTPL

Nucleotide

• Length: 474 bp

>T161981 NZ_CP071441:3139216-3139527 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16190.45 Da
• Isoelectric Point: 4.9405

>AT161981 WP_004862777.1 NZ_CP055216:c2497151-2496708 [Raoultella ornithinolytica]
MKTWVPAEQPADNALWRYAGLPANRGMQLIELLSMGLPVSILDNIHEWTEMSKTDILRITGINERNVARRKSAGGTLTPG
ESERVARFVRVLDTAVDYFGSKQDAYDWLQSPVRGLGNVAPIDLIATESGALEVTDLIGRLEHGVFA

Nucleotide

• Length: 444 bp

>AT161981 NZ_CP071441:3139528-3139818 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A808Y7A4