Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 74698..75245 Replicon chromosome
Accession NZ_CP055216
Organism Raoultella ornithinolytica strain STEFF_15

Toxin (Protein)


Gene name ccdB Uniprot ID A0A808XUV0
Locus tag HUZ43_RS00350 Protein ID WP_048025274.1
Coordinates 74698..75006 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A808XYT1
Locus tag HUZ43_RS00355 Protein ID WP_004869059.1
Coordinates 75009..75245 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ43_RS00320 (HUZ43_00320) 69886..70626 + 741 WP_015585491.1 4-oxalmesaconate hydratase -
HUZ43_RS00325 (HUZ43_00325) 70623..71336 + 714 WP_015585492.1 4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase -
HUZ43_RS00330 (HUZ43_00330) 71341..72432 + 1092 WP_015585493.1 4-oxalomesaconate tautomerase -
HUZ43_RS00335 (HUZ43_00335) 72429..73295 + 867 WP_015585494.1 DUF1932 domain-containing protein -
HUZ43_RS00340 (HUZ43_00340) 73296..73586 - 291 WP_044350974.1 YicS family protein -
HUZ43_RS00345 (HUZ43_00345) 73680..74582 - 903 WP_185762445.1 EamA family transporter -
HUZ43_RS00350 (HUZ43_00350) 74698..75006 - 309 WP_048025274.1 CcdB family protein Toxin
HUZ43_RS00355 (HUZ43_00355) 75009..75245 - 237 WP_004869059.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HUZ43_RS00360 (HUZ43_00360) 75351..76784 - 1434 WP_069476354.1 PTS N-acetylmuramic acid transporter subunit IIBC -
HUZ43_RS00365 (HUZ43_00365) 76809..77711 - 903 WP_044350979.1 N-acetylmuramic acid 6-phosphate etherase -
HUZ43_RS00370 (HUZ43_00370) 77876..79039 - 1164 WP_004869055.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11778.62 Da        Isoelectric Point: 6.7156

>T161971 WP_048025274.1 NZ_CP055216:c75006-74698 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRVVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Download         Length: 309 bp

>T161971 NZ_CP071441:c627136-625814 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8898.08 Da        Isoelectric Point: 5.6579

>AT161971 WP_004869059.1 NZ_CP055216:c75245-75009 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT161971 NZ_CP071441:c627402-627136 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808XUV0

Antitoxin

Source ID Structure
AlphaFold DB A0A808XYT1

References