Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 126531..127270 Replicon plasmid unnamed1
Accession NZ_CP055214
Organism Klebsiella quasipneumoniae strain STEFF_21

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag HUZ48_RS25545 Protein ID WP_013087172.1
Coordinates 126531..127016 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag HUZ48_RS25550 Protein ID WP_012540086.1
Coordinates 127004..127270 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ48_RS25495 (HUZ48_25435) 122132..122545 - 414 WP_004182074.1 helix-turn-helix domain-containing protein -
HUZ48_RS25500 (HUZ48_25440) 122546..122824 - 279 WP_004152721.1 helix-turn-helix transcriptional regulator -
HUZ48_RS25505 (HUZ48_25445) 122814..123134 - 321 WP_004152720.1 type II toxin-antitoxin system RelE/ParE family toxin -
HUZ48_RS25510 (HUZ48_25450) 123215..123439 - 225 WP_004152719.1 hypothetical protein -
HUZ48_RS25515 (HUZ48_25455) 123450..123662 - 213 WP_004152718.1 hypothetical protein -
HUZ48_RS25520 (HUZ48_25460) 123723..124079 - 357 WP_237902772.1 hypothetical protein -
HUZ48_RS25525 (HUZ48_25465) 124715..125065 - 351 WP_023320095.1 hypothetical protein -
HUZ48_RS25530 (HUZ48_25470) 125062..125334 - 273 WP_004182067.1 hypothetical protein -
HUZ48_RS25535 (HUZ48_25475) 126030..126152 - 123 WP_085842394.1 Hok/Gef family protein -
HUZ48_RS25540 126100..126249 - 150 Protein_147 DUF5431 family protein -
HUZ48_RS25545 (HUZ48_25480) 126531..127016 - 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
HUZ48_RS25550 (HUZ48_25485) 127004..127270 - 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
HUZ48_RS25555 (HUZ48_25490) 127569..127892 + 324 WP_012540250.1 metalloregulator ArsR/SmtB family transcription factor -
HUZ48_RS25560 (HUZ48_25495) 127894..128607 + 714 WP_012540184.1 arsenical resistance protein ArsH -
HUZ48_RS25565 (HUZ48_25500) 128616..129161 + 546 WP_012540227.1 sigma-70 family RNA polymerase sigma factor -
HUZ48_RS25570 (HUZ48_25505) 129237..129599 + 363 WP_094716534.1 arsenic metallochaperone ArsD family protein -
HUZ48_RS25575 (HUZ48_25510) 129626..131377 + 1752 WP_162866166.1 arsenical pump-driving ATPase -
HUZ48_RS25580 (HUZ48_25515) 131502..132038 + 537 WP_012540238.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia / sul2 / floR / catA2 / qnrS1 - 1..160169 160169


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T161970 WP_013087172.1 NZ_CP055214:c127016-126531 [Klebsiella quasipneumoniae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T161970 NZ_CP071441:532360-532536 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT161970 WP_012540086.1 NZ_CP055214:c127270-127004 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT161970 NZ_CP071441:532582-532998 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References