Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3939705..3940324 Replicon chromosome
Accession NZ_CP055213
Organism Klebsiella quasipneumoniae strain STEFF_21

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUZ48_RS19025 Protein ID WP_002892050.1
Coordinates 3940106..3940324 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUZ48_RS19020 Protein ID WP_002892066.1
Coordinates 3939705..3940079 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ48_RS19010 (3934859) 3934859..3936052 + 1194 WP_023288645.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ48_RS19015 (3936075) 3936075..3939221 + 3147 WP_023288644.1 multidrug efflux RND transporter permease subunit AcrB -
HUZ48_RS19020 (3939705) 3939705..3940079 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUZ48_RS19025 (3940106) 3940106..3940324 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HUZ48_RS19030 (3940483) 3940483..3941049 + 567 WP_023288643.1 maltose O-acetyltransferase -
HUZ48_RS19035 (3941021) 3941021..3941152 - 132 WP_032428629.1 hypothetical protein -
HUZ48_RS19040 (3941186) 3941186..3941656 + 471 WP_023288642.1 YlaC family protein -
HUZ48_RS19045 (3941625) 3941625..3943082 - 1458 WP_237902201.1 PLP-dependent aminotransferase family protein -
HUZ48_RS19050 (3943183) 3943183..3943881 + 699 WP_110243759.1 GNAT family protein -
HUZ48_RS19055 (3943878) 3943878..3944018 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HUZ48_RS19060 (3944018) 3944018..3944281 - 264 WP_004204754.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161967 WP_002892050.1 NZ_CP055213:3940106-3940324 [Klebsiella quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T161967 NZ_CP071440:c97525-96995 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT161967 WP_002892066.1 NZ_CP055213:3939705-3940079 [Klebsiella quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT161967 NZ_CP071440:c97798-97529 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References