Detailed information of TA system TA161966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2562415..2564003
• Replicon: chromosome
• Accession: NZ_CP055213
• Organism: Klebsiella quasipneumoniae strain STEFF_21

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HUZ48_RS12420
• Protein ID: WP_072420212.1
• Coordinates: 2562415..2563737 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2N4W0X3
• Locus tag: HUZ48_RS12425
• Protein ID: WP_023289667.1
• Coordinates: 2563737..2564003 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ48_RS12405 (2557471)	2557471..2559972	+	2502	WP_237902716.1	fimbria/pilus outer membrane usher protein	-
HUZ48_RS12410 (2559960)	2559960..2560964	+	1005	WP_110211330.1	fimbrial protein	-
HUZ48_RS12415 (2561106)	2561106..2562398	+	1293	WP_032452829.1	glycoside hydrolase family 10 protein	-
HUZ48_RS12420 (2562415)	2562415..2563737	-	1323	WP_072420212.1	type II toxin-antitoxin system HipA family toxin	Toxin
HUZ48_RS12425 (2563737)	2563737..2564003	-	267	WP_023289667.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HUZ48_RS12430 (2564389)	2564389..2564715	-	327	WP_072420213.1	hydroxyisourate hydrolase	-
HUZ48_RS12435 (2564712)	2564712..2565212	-	501	WP_072420214.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
HUZ48_RS12440 (2565190)	2565190..2566569	-	1380	WP_032452824.1	nucleobase:cation symporter-2 family protein	-
HUZ48_RS12445 (2566915)	2566915..2568069	+	1155	WP_032452823.1	FAD-dependent urate hydroxylase HpxO	-
HUZ48_RS12450 (2568050)	2568050..2568973	-	924	WP_032452822.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2339577..2597132	257555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49209.53 Da
• Isoelectric Point: 8.6110

>T161966 WP_072420212.1 NZ_CP055213:c2563737-2562415 [Klebsiella quasipneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDDEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATRGRKTEINAIYPRHFLATAKAVNFPRDQMLAILDEFADRVP
QAIESARRTLPADFSPHVWRAITENMLKLHARLQQGLQAR

Nucleotide

• Length: 1323 bp

>T161966 NZ_CP071440:96497-96772 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGAAGACTGGGTAAATTAACATCGCCCACACTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.25 Da
• Isoelectric Point: 7.3163

>AT161966 WP_023289667.1 NZ_CP055213:c2564003-2563737 [Klebsiella quasipneumoniae]
MHSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETATAP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT161966 NZ_CP071440:96178-96408 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTCG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N4W0X3