Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 32887..33409 Replicon plasmid unnamed2
Accession NZ_CP055212
Organism Klebsiella pneumoniae strain STEFF_2

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag HUZ38_RS27355 Protein ID WP_000220561.1
Coordinates 33128..33409 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag HUZ38_RS27350 Protein ID WP_000121743.1
Coordinates 32887..33138 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ38_RS27330 (28302) 28302..28532 + 231 WP_000051066.1 plasmid partition protein ParG -
HUZ38_RS27335 (28956) 28956..30020 + 1065 WP_012291478.1 ParA family protein -
HUZ38_RS27340 (30282) 30282..30470 + 189 WP_000957857.1 hypothetical protein -
HUZ38_RS27345 (30480) 30480..31679 + 1200 WP_000948429.1 IS91 family transposase -
HUZ38_RS27350 (32887) 32887..33138 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
HUZ38_RS27355 (33128) 33128..33409 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ38_RS27360 (33648) 33648..33740 - 93 Protein_38 IS6 family transposase -
HUZ38_RS27365 (33906) 33906..35105 - 1200 WP_000948429.1 IS91 family transposase -
HUZ38_RS27370 (35115) 35115..35303 - 189 WP_000957857.1 hypothetical protein -
HUZ38_RS27375 (35423) 35423..36853 + 1431 Protein_41 IS4-like element IS50R family transposase -
HUZ38_RS27380 (36882) 36882..37676 + 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
HUZ38_RS27385 (37697) 37697..37933 + 237 Protein_43 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / sul2 / aph(3')-IIa / blaTEM-1B / rmtB / blaCTX-M-55 - 1..102776 102776
- inside IScluster/Tn aph(3')-IIa / blaTEM-1B / rmtB / blaCTX-M-55 - 30480..45923 15443


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T161952 WP_000220561.1 NZ_CP055212:33128-33409 [Klebsiella pneumoniae]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T161952 NZ_CP071439:3394875-3395225 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT161952 WP_000121743.1 NZ_CP055212:32887-33138 [Klebsiella pneumoniae]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT161952 NZ_CP071439:3394630-3394881 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References