Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 177279..178024 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP055211 | ||
| Organism | Klebsiella pneumoniae strain STEFF_2 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | A0A331KSM6 |
| Locus tag | HUZ38_RS26915 | Protein ID | WP_032408901.1 |
| Coordinates | 177533..178024 (+) | Length | 164 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | - |
| Locus tag | HUZ38_RS26910 | Protein ID | WP_014386183.1 |
| Coordinates | 177279..177545 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUZ38_RS26860 (HUZ38_26765) | 172831..173244 | - | 414 | WP_013023817.1 | helix-turn-helix domain-containing protein | - |
| HUZ38_RS26865 (HUZ38_26770) | 173245..173523 | - | 279 | WP_004152721.1 | helix-turn-helix transcriptional regulator | - |
| HUZ38_RS26870 (HUZ38_26775) | 173513..173833 | - | 321 | WP_014386190.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HUZ38_RS26875 (HUZ38_26780) | 173914..174138 | - | 225 | WP_014386189.1 | hypothetical protein | - |
| HUZ38_RS26880 (HUZ38_26785) | 174149..174361 | - | 213 | WP_014386188.1 | hypothetical protein | - |
| HUZ38_RS26885 (HUZ38_26790) | 174423..174749 | - | 327 | WP_014386187.1 | hypothetical protein | - |
| HUZ38_RS26890 (HUZ38_26795) | 175386..175736 | - | 351 | WP_014386186.1 | hypothetical protein | - |
| HUZ38_RS26895 (HUZ38_26800) | 175733..176005 | - | 273 | WP_032408902.1 | hypothetical protein | - |
| HUZ38_RS26900 (HUZ38_26805) | 176195..176680 | + | 486 | WP_014386185.1 | hypothetical protein | - |
| HUZ38_RS26905 (HUZ38_26810) | 176924..177082 | - | 159 | WP_014386184.1 | type I toxin-antitoxin system Hok family toxin | - |
| HUZ38_RS26910 (HUZ38_26815) | 177279..177545 | + | 267 | WP_014386183.1 | DUF1778 domain-containing protein | Antitoxin |
| HUZ38_RS26915 (HUZ38_26820) | 177533..178024 | + | 492 | WP_032408901.1 | GNAT family N-acetyltransferase | Toxin |
| HUZ38_RS26920 (HUZ38_26825) | 178465..178716 | - | 252 | WP_032408900.1 | aldo/keto reductase | - |
| HUZ38_RS26925 (HUZ38_26830) | 178913..180505 | - | 1593 | Protein_199 | IS66 family transposase | - |
| HUZ38_RS26930 (HUZ38_26835) | 180536..180886 | - | 351 | WP_014386180.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| HUZ38_RS26935 (HUZ38_26840) | 180883..181323 | - | 441 | WP_014386179.1 | transposase | - |
| HUZ38_RS26940 (HUZ38_26845) | 181585..182340 | - | 756 | WP_032408898.1 | DeoR/GlpR family DNA-binding transcription regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | aph(3')-Ia / tet(A) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 | - | 1..223722 | 223722 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 164 a.a. Molecular weight: 17667.46 Da Isoelectric Point: 8.8757
>T161950 WP_032408901.1 NZ_CP055211:177533-178024 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT
Download Length: 492 bp
>T161950 NZ_CP071439:c2663352-2663167 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCTATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA
GTGAAAAGTGCAGACGTGATCGCTATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA
Antitoxin
Download Length: 89 a.a. Molecular weight: 10090.66 Da Isoelectric Point: 5.1169
>AT161950 WP_014386183.1 NZ_CP055211:177279-177545 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK
Download Length: 267 bp
>AT161950 NZ_CP071439:c2663150-2662740 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|