Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4311998..4312617 Replicon chromosome
Accession NZ_CP055210
Organism Klebsiella pneumoniae strain STEFF_2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUZ38_RS20815 Protein ID WP_002892050.1
Coordinates 4311998..4312216 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUZ38_RS20820 Protein ID WP_002892066.1
Coordinates 4312243..4312617 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ38_RS20780 (4308045) 4308045..4308308 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
HUZ38_RS20785 (4308308) 4308308..4308448 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HUZ38_RS20790 (4308445) 4308445..4309143 - 699 WP_002892021.1 GNAT family protein -
HUZ38_RS20795 (4309244) 4309244..4310695 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HUZ38_RS20800 (4310670) 4310670..4311140 - 471 WP_002892026.1 YlaC family protein -
HUZ38_RS20805 (4311161) 4311161..4311301 + 141 WP_004147370.1 hypothetical protein -
HUZ38_RS20810 (4311273) 4311273..4311839 - 567 WP_002892030.1 maltose O-acetyltransferase -
HUZ38_RS20815 (4311998) 4311998..4312216 - 219 WP_002892050.1 HHA domain-containing protein Toxin
HUZ38_RS20820 (4312243) 4312243..4312617 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUZ38_RS20825 (4313103) 4313103..4316249 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HUZ38_RS20830 (4316272) 4316272..4317465 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161947 WP_002892050.1 NZ_CP055210:c4312216-4311998 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T161947 NZ_CP071439:1974465-1974842 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT161947 WP_002892066.1 NZ_CP055210:c4312617-4312243 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT161947 NZ_CP071439:1974008-1974388 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGTTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References