Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3054692..3055317 Replicon chromosome
Accession NZ_CP055210
Organism Klebsiella pneumoniae strain STEFF_2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ38_RS14910 Protein ID WP_002882817.1
Coordinates 3054934..3055317 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ38_RS14905 Protein ID WP_004150355.1
Coordinates 3054692..3054934 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ38_RS14880 (3050683) 3050683..3051282 + 600 WP_004151865.1 glucose-1-phosphatase -
HUZ38_RS14885 (3051276) 3051276..3052136 + 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ38_RS14890 (3052133) 3052133..3052570 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ38_RS14895 (3052615) 3052615..3053556 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ38_RS14900 (3053570) 3053570..3054487 - 918 WP_004181612.1 alpha/beta hydrolase -
HUZ38_RS14905 (3054692) 3054692..3054934 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ38_RS14910 (3054934) 3054934..3055317 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ38_RS14915 (3055491) 3055491..3056420 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ38_RS14920 (3056417) 3056417..3057052 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ38_RS14925 (3057049) 3057049..3057951 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T161942 WP_002882817.1 NZ_CP055210:3054934-3055317 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T161942 NZ_CP071439:221129-221305 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCAATGTAGCAAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTACGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT161942 WP_004150355.1 NZ_CP055210:3054692-3054934 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT161942 NZ_CP071439:221351-221767 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGACATCACTCAGCAAGAATTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References