Detailed information of TA system TA161925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1981..2540
• Replicon: plasmid unnamed3
• Accession: NZ_CP055202
• Organism: Shigella flexneri strain STEFF_3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: HUZ39_RS25370
• Protein ID: WP_000421259.1
• Coordinates: 2265..2540 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: HUZ39_RS25365
• Protein ID: WP_001178087.1
• Coordinates: 1981..2265 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ39_RS25360 (46)	46..1077	+	1032	WP_239637986.1	plasmid replication initiator RepA	-
HUZ39_RS25365 (1981)	1981..2265	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
HUZ39_RS25370 (2265)	2265..2540	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ39_RS25375 (2646)	2646..2927	+	282	WP_001105066.1	hypothetical protein	-
HUZ39_RS25380 (3119)	3119..3385	-	267	WP_000627832.1	hypothetical protein	-
HUZ39_RS25385 (3426)	3426..3572	-	147	Protein_5	Tn3 family transposase	-
HUZ39_RS25390 (3774)	3774..4973	-	1200	WP_000948429.1	IS91 family transposase	-
HUZ39_RS25395 (4983)	4983..5171	-	189	WP_000957857.1	hypothetical protein	-
HUZ39_RS25400 (5290)	5290..5715	-	426	Protein_8	recombinase family protein	-
HUZ39_RS25405 (6025)	6025..6561	+	537	Protein_9	PAS domain-containing protein	-
HUZ39_RS25410 (6629)	6629..7084	-	456	Protein_10	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-24	-	1..91691	91691

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T161925 WP_000421259.1 NZ_CP055202:2265-2540 [Shigella flexneri]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T161925 NZ_CP071436:968156-968491 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT161925 WP_001178087.1 NZ_CP055202:1981-2265 [Shigella flexneri]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT161925 NZ_CP071436:967908-968156 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625