Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 61817..62418 Replicon plasmid unnamed2
Accession NZ_CP055201
Organism Shigella flexneri strain STEFF_3

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag HUZ39_RS25130 Protein ID WP_001216034.1
Coordinates 61817..62197 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag HUZ39_RS25135 Protein ID WP_001190712.1
Coordinates 62197..62418 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ39_RS25100 56943..57044 - 102 Protein_53 transcriptional regulator -
HUZ39_RS25105 (HUZ39_24930) 57258..58742 - 1485 WP_000124163.1 hypothetical protein -
HUZ39_RS25110 (HUZ39_24935) 58742..59935 - 1194 WP_074478412.1 terminase -
HUZ39_RS25115 (HUZ39_24940) 60021..60473 - 453 WP_001326849.1 late promoter-activating protein -
HUZ39_RS25120 (HUZ39_24945) 60562..61605 - 1044 WP_001561086.1 DUF968 domain-containing protein -
HUZ39_RS25125 (HUZ39_24950) 61633..61812 - 180 WP_000113018.1 hypothetical protein -
HUZ39_RS25130 (HUZ39_24955) 61817..62197 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HUZ39_RS25135 (HUZ39_24960) 62197..62418 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HUZ39_RS25140 (HUZ39_24965) 62491..62880 - 390 WP_000506726.1 S24 family peptidase -
HUZ39_RS25145 (HUZ39_24970) 63004..63255 - 252 WP_001283837.1 DNA polymerase III subunit theta -
HUZ39_RS25150 63288..63638 + 351 WP_000551789.1 hypothetical protein -
HUZ39_RS25155 (HUZ39_24985) 63869..64480 - 612 Protein_64 hypothetical protein -
HUZ39_RS25160 (HUZ39_24990) 64462..64836 - 375 WP_033553822.1 hypothetical protein -
HUZ39_RS25165 (HUZ39_24995) 64843..65136 - 294 WP_033553821.1 hypothetical protein -
HUZ39_RS25170 (HUZ39_25000) 65315..65548 - 234 WP_000517420.1 hypothetical protein -
HUZ39_RS25175 (HUZ39_25005) 65625..65885 - 261 WP_033553819.1 hypothetical protein -
HUZ39_RS25180 (HUZ39_25010) 65882..66802 - 921 WP_089046483.1 DUF551 domain-containing protein -
HUZ39_RS25185 (HUZ39_25015) 66804..67295 - 492 WP_089046484.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..92910 92910


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T161924 WP_001216034.1 NZ_CP055201:c62197-61817 [Shigella flexneri]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T161924 NZ_CP071436:844084-844491 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT161924 WP_001190712.1 NZ_CP055201:c62418-62197 [Shigella flexneri]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT161924 NZ_CP071436:843837-844103 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References