Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4386916..4387413 Replicon chromosome
Accession NZ_CP055199
Organism Shigella flexneri strain STEFF_3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUZ39_RS21485 Protein ID WP_001173343.1
Coordinates 4386916..4387089 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUZ39_RS21490 Protein ID WP_000398619.1
Coordinates 4387117..4387413 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ39_RS21475 (4383586) 4383586..4385043 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HUZ39_RS21480 (4385280) 4385280..4386797 + 1518 WP_001295074.1 lysine--tRNA ligase -
HUZ39_RS21485 (4386916) 4386916..4387089 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUZ39_RS21490 (4387117) 4387117..4387413 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUZ39_RS21495 (4387640) 4387640..4387912 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
HUZ39_RS21500 (4387924) 4387924..4388154 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
HUZ39_RS21505 (4388335) 4388335..4389966 + 1632 WP_001216487.1 sensor histidine kinase -
HUZ39_RS21510 (4389963) 4389963..4390682 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T161917 WP_001173343.1 NZ_CP055199:c4387089-4386916 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T161917 NZ_CP071409:1815629-1815716 [[Pantoea] beijingensis]
GCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTACCAAGAGCCATTCCCCTGGACCGAATACAGGAATCGTATTCGGT
CTTTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT161917 WP_000398619.1 NZ_CP055199:c4387413-4387117 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT161917 NZ_CP071409:c1815716-1815609 [[Pantoea] beijingensis]
AAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGGAATGGCTCTTGGTAGAGCCGTGCGCTAAAAGTTGGCATTA
ATGCAGGCGAGGTCGCCATGCCCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References