Detailed information of TA system TA161914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3636169..3637006
• Replicon: chromosome
• Accession: NZ_CP055199
• Organism: Shigella flexneri strain STEFF_3

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: HUZ39_RS17860
• Protein ID: WP_000227784.1
• Coordinates: 3636464..3637006 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: HUZ39_RS17855
• Protein ID: WP_001297137.1
• Coordinates: 3636169..3636480 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ39_RS17830 (3631189)	3631189..3632136	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
HUZ39_RS17835 (3632158)	3632158..3634149	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HUZ39_RS17840 (3634139)	3634139..3634753	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HUZ39_RS17845 (3634753)	3634753..3635082	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HUZ39_RS17850 (3635094)	3635094..3635984	+	891	WP_000971336.1	heme o synthase	-
HUZ39_RS17855 (3636169)	3636169..3636480	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
HUZ39_RS17860 (3636464)	3636464..3637006	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
HUZ39_RS17865 (3637062)	3637062..3637997	-	936	WP_001365761.1	tetratricopeptide repeat protein	-
HUZ39_RS17870 (3638405)	3638405..3639769	+	1365	WP_001000974.1	MFS transporter	-
HUZ39_RS17875 (3639897)	3639897..3640388	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HUZ39_RS17880 (3640556)	3640556..3641467	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T161914 WP_000227784.1 NZ_CP055199:3636464-3637006 [Shigella flexneri]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T161914 NZ_CP071405:2967889-2968104 [Pantoea deleyi]
ATGAACAAAACCCTGACAAAAACCGATTATTTGATGCGGCTGCGACGCTGCCGCTCGCTGGATACCCTGGAAAGGGTGAT
TGAAAAGAATAAGTATGAATTACCGGAAGCGGAATTAGCCATATTCTATTCGGCGGCTGACCATCGTCTGGCGGAACTGA
CGATGAACAAGCTCTACGATAAAGTGCCGGGTTCAGTGTGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT161914 WP_001297137.1 NZ_CP055199:3636169-3636480 [Shigella flexneri]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT161914 NZ_CP071405:2967487-2967864 [Pantoea deleyi]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACCTGTGTGAGAGTCTGTTTGACGACAGCATGGC
ATCATTAACTGACAGCCATCACGGCTGGGTAAATGATCCCACTTCCGAAAGCAATCTGCAGCTTAATGATTTGATTGAGC
ATATCGCCTCTTTCACCATGAATTACAAAATCAAGCACGTTGAAGATGAAGCGCTGATTTCGCAAATCGATGAATATCTT
GACGATACTTTTATGCTATTCAGCAACTATGGTGTCACAAGTCCGGATCTTCAGCGGTGGCAACGTTCAGCGAAACGCTT
ATTTAATCTGTTCACTGAAGAGTGCGCTTTTCTCCAGCAGCCAAGCCATTCATTATAG

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3