Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3602093..3602711 Replicon chromosome
Accession NZ_CP055199
Organism Shigella flexneri strain STEFF_3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ39_RS17690 Protein ID WP_001291435.1
Coordinates 3602493..3602711 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ39_RS17685 Protein ID WP_000344800.1
Coordinates 3602093..3602467 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ39_RS17675 (3597182) 3597182..3598375 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ39_RS17680 (3598398) 3598398..3601547 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ39_RS17685 (3602093) 3602093..3602467 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ39_RS17690 (3602493) 3602493..3602711 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ39_RS17695 (3602882) 3602882..3603433 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ39_RS17700 (3603549) 3603549..3604019 + 471 WP_000136192.1 YlaC family protein -
HUZ39_RS17705 (3604183) 3604183..3605733 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ39_RS17710 (3605775) 3605775..3606128 - 354 WP_000878141.1 DUF1428 family protein -
HUZ39_RS17720 (3606507) 3606507..3606818 + 312 WP_000409911.1 MGMT family protein -
HUZ39_RS17725 (3606849) 3606849..3607421 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161913 WP_001291435.1 NZ_CP055199:3602493-3602711 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161913 NZ_CP071405:c2094240-2094058 [Pantoea deleyi]
ATGAAGTACAGTGAATTCAGACGCTGGCTCATTCAGCAAGGCGCTATTCTGGTTAAGCACCGCAGAGGAAGCAGCCATTT
CAAAGTCTTCTCAGGCGACAGGCAGTCTGTCTTTCCGGATCATGGCGCGAAAGAAATGCCGGAAGGGACGCGCAAGAAAA
TTCTAAAAGATCCGGGCCTGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161913 WP_000344800.1 NZ_CP055199:3602093-3602467 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161913 NZ_CP071405:c2094035-2093610 [Pantoea deleyi]
ATGTTTAACTATGCCGTCAGATTAACGCATGACCTGAAAACCGATAGCTGGGAGGTGTCATGTCGCGATTTACCCTTACT
GCATTCAGTTGGCGACACCGTTGAGGAAGCGCTGGCCGAGGCAGCAGATGGACTGGTAGCCGCTATCTTTCTGGAAATGG
ATGCCCGTCGTCCCATCCCGTTAGCAAGTGATGCGCAGCAGGGGGAGTACATCGTTTCTGTGCCTGTCCTGGTAGCGATG
AAAGCGGCACTGTATAACGCCATGATTGAAACCGGTACCCGCAAGGCAGATCTGGCACGCCAGCTGGGTGTAAATGGACC
ACAGGTTGATCGACTGCTTGACGTAGAGCACTCGTCAAAAGTTGAAAAAGTTGAACTGGCGCTGGCGCAGCTGGGCCGTA
AAGCCCGGGTTGAGATTCTCATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References