Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1955660..1956491 Replicon chromosome
Accession NZ_CP055199
Organism Shigella flexneri strain STEFF_3

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag HUZ39_RS09455 Protein ID WP_000854814.1
Coordinates 1955660..1956034 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag HUZ39_RS09460 Protein ID WP_001285585.1
Coordinates 1956123..1956491 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ39_RS09415 (1951056) 1951056..1952222 + 1167 WP_000830163.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
HUZ39_RS09420 (1952341) 1952341..1952814 + 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
HUZ39_RS09425 (1953012) 1953012..1954070 + 1059 WP_001200905.1 FUSC family protein -
HUZ39_RS09430 (1954242) 1954242..1954571 + 330 WP_000450409.1 DUF496 family protein -
HUZ39_RS09435 (1954672) 1954672..1954806 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
HUZ39_RS09440 (1954926) 1954926..1955054 + 129 Protein_1854 transposase domain-containing protein -
HUZ39_RS09445 (1955343) 1955343..1955423 - 81 Protein_1855 hypothetical protein -
HUZ39_RS09450 (1955469) 1955469..1955663 - 195 WP_000988600.1 DUF5983 family protein -
HUZ39_RS09455 (1955660) 1955660..1956034 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
HUZ39_RS09460 (1956123) 1956123..1956491 - 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
HUZ39_RS09465 (1956565) 1956565..1956786 - 222 WP_000692323.1 DUF987 domain-containing protein -
HUZ39_RS09470 (1956849) 1956849..1957325 - 477 WP_001186773.1 RadC family protein -
HUZ39_RS09475 (1957341) 1957341..1957820 - 480 WP_000860076.1 antirestriction protein -
HUZ39_RS09480 (1957902) 1957902..1958723 - 822 WP_001234530.1 DUF932 domain-containing protein -
HUZ39_RS09485 (1958944) 1958944..1959354 - 411 WP_000846713.1 hypothetical protein -
HUZ39_RS09490 (1959370) 1959370..1960052 - 683 Protein_1864 hypothetical protein -
HUZ39_RS09495 (1960188) 1960188..1961258 - 1071 WP_000102643.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1935222..1962160 26938


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T161902 WP_000854814.1 NZ_CP055199:c1956034-1955660 [Shigella flexneri]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T161902 NZ_CP071399:1130028-1130441 [Rhizobium leguminosarum]
ATGACGGCCTGGATGCTCGATACCAACATGGTGAGCCATGTCATCAAGGGCGATCGGCCGGAGATCATCGAAAGGTTGGC
CGCGCTTCCGATCGAGGAGATCGTCATCTCATCGATCACCGAAGGGGAATTGTTCTACGGTCTTGCCAAGCGAGGCTATC
CGAAGGCGCTGTCGGAACGGATCCGCCAATTCCTGCTCCGGGTCGACGTTCTTCCTTGGGATCACGATGTCACGCGAACT
CATGGCGACCTGCGCGCTGCCTGTGACGCCAAAGGGGTGACTCTGGCGCCCCTCGATATGATGATTGCCGCTCATGCCGT
TGCAACTGCCGCAACCCTCGTCACGCGCGACAAGGCTTTTGCTCGCGTTTCGGAACCTTTGAAGGTCGATGACTGGGCGA
ATGAAGGAAGCTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT161902 WP_001285585.1 NZ_CP055199:c1956491-1956123 [Shigella flexneri]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT161902 NZ_CP071399:1129768-1130031 [Rhizobium leguminosarum]
ATGGCAAAGAGAACAGCCAAGATTTTTACGACCGGCCGCAGCCAGGCAGTGAGGCTCCCTGCGGAGTTCCGCTTCGAGGA
GAGCGAGGTGTTCGTCCGTCGTGATCCGAAGTCCGGCGATGTCATTCTGTCACGCAAGCCGAATTCGTGGGATGGTCTCT
TTGAGCTTTATGGCAAGGATCGGGTTCCGGACGATTTCCTGGGGCCAGACGACCGCCGCCAGCCTTCGCATGATCGTGAT
CCATTCGAGGGCTGGAAGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References