Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1077526..1078109 Replicon chromosome
Accession NZ_CP055199
Organism Shigella flexneri strain STEFF_3

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag HUZ39_RS05220 Protein ID WP_000254745.1
Coordinates 1077774..1078109 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HUZ39_RS05215 Protein ID WP_000581937.1
Coordinates 1077526..1077774 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ39_RS05205 (1073865) 1073865..1075166 + 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
HUZ39_RS05210 (1075214) 1075214..1077448 + 2235 WP_000226815.1 GTP pyrophosphokinase -
HUZ39_RS05215 (1077526) 1077526..1077774 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HUZ39_RS05220 (1077774) 1077774..1078109 + 336 WP_000254745.1 endoribonuclease MazF Toxin
HUZ39_RS05225 (1078180) 1078180..1078971 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HUZ39_RS05230 (1079199) 1079199..1080836 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HUZ39_RS05235 (1080924) 1080924..1082222 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T161899 WP_000254745.1 NZ_CP055199:1077774-1078109 [Shigella flexneri]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T161899 NZ_CP071395:c35438-35133 [Klebsiella michiganensis]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGCGACATCATTGATAC
TCCCGGGCGGAGAATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGCGAGCTCTATCCGGTGG
TGCACATCGGGGACGACAGCTATCGCCTGATGACCACGGATATGGCCAGCGTCACGGCCTTGGTCACCGGGGAGGAAGTG
ACCGATCTCAGCCATCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT161899 WP_000581937.1 NZ_CP055199:1077526-1077774 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT161899 NZ_CP071395:c35658-35440 [Klebsiella michiganensis]
ATGAAACAGCGTATTACTGTCACCATTGACAGCGACAACTATCAGTTGCTTAAAGCGGCTGACATCAATATCTCAGGTCT
GGTCAATACGGCCATGCAAAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCGGAAAATCAGGAAGGGATGGCCG
AAGTCGCTCGGTTTATTGATATGAACGGTTCATTTGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References