Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 941191..941845 Replicon chromosome
Accession NZ_CP055199
Organism Shigella flexneri strain STEFF_3

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HUZ39_RS04605 Protein ID WP_000244772.1
Coordinates 941438..941845 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ39_RS04600 Protein ID WP_000354046.1
Coordinates 941191..941457 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ39_RS04575 (936479) 936479..937222 + 744 WP_000951964.1 SDR family oxidoreductase -
HUZ39_RS04580 (937279) 937279..938712 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
HUZ39_RS04585 (938757) 938757..939068 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HUZ39_RS04590 (939232) 939232..939891 + 660 WP_000250274.1 hemolysin III family protein -
HUZ39_RS04595 (939968) 939968..940948 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HUZ39_RS04600 (941191) 941191..941457 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ39_RS04605 (941438) 941438..941845 + 408 WP_000244772.1 protein YgfX Toxin
HUZ39_RS04610 (941885) 941885..942406 - 522 WP_001055874.1 flavodoxin FldB -
HUZ39_RS04615 (942518) 942518..943414 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ39_RS04620 (943439) 943439..944149 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ39_RS04625 (944155) 944155..945888 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T161898 WP_000244772.1 NZ_CP055199:941438-941845 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T161898 NZ_CP071394:174767-175051 [Klebsiella michiganensis]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT161898 WP_000354046.1 NZ_CP055199:941191-941457 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT161898 NZ_CP071394:174529-174777 [Klebsiella michiganensis]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References