Detailed information of TA system TA161897

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 826766..827607
• Replicon: chromosome
• Accession: NZ_CP055199
• Organism: Shigella flexneri strain STEFF_3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0WJM1
• Locus tag: HUZ39_RS04005
• Protein ID: WP_000854822.1
• Coordinates: 826766..827149 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: HUZ39_RS04010
• Protein ID: WP_001285610.1
• Coordinates: 827239..827607 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ39_RS03975 (822412)	822412..824253	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
HUZ39_RS03980 (824331)	824331..824837	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
HUZ39_RS03990 (825136)	825136..825981	-	846	WP_001280435.1	DUF4942 domain-containing protein	-
HUZ39_RS03995 (826078)	826078..826275	-	198	WP_000839263.1	DUF957 domain-containing protein	-
HUZ39_RS04000 (826287)	826287..826775	-	489	WP_001177592.1	DUF5983 family protein	-
HUZ39_RS04005 (826766)	826766..827149	-	384	WP_000854822.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
HUZ39_RS04010 (827239)	827239..827607	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUZ39_RS04015 (827687)	827687..827908	-	222	WP_000692350.1	DUF987 domain-containing protein	-
HUZ39_RS04020 (827977)	827977..828453	-	477	WP_072661507.1	RadC family protein	-
HUZ39_RS04025 (828469)	828469..828954	-	486	WP_072794436.1	antirestriction protein	-
HUZ39_RS04030 (829045)	829045..829863	-	819	WP_001234702.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14295.32 Da
• Isoelectric Point: 8.2830

>T161897 WP_000854822.1 NZ_CP055199:c827149-826766 [Shigella flexneri]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Nucleotide

• Length: 384 bp

>T161897 NZ_CP071394:82675-83157 [Klebsiella michiganensis]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT161897 WP_001285610.1 NZ_CP055199:c827607-827239 [Shigella flexneri]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT161897 NZ_CP071394:82406-82684 [Klebsiella michiganensis]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• AlphaFold DB: A0A1M0WJM1