Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 337059..337690 Replicon chromosome
Accession NZ_CP055199
Organism Shigella flexneri strain STEFF_3

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag HUZ39_RS01540 Protein ID WP_001259388.1
Coordinates 337059..337334 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HUZ39_RS01545 Protein ID WP_000593555.1
Coordinates 337331..337690 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ39_RS01525 (332063) 332063..333130 + 1068 WP_000361485.1 HlyD family secretion protein -
HUZ39_RS01530 (333127) 333127..335862 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
HUZ39_RS01535 (335862) 335862..336986 + 1125 WP_001314210.1 ABC transporter permease -
HUZ39_RS01540 (337059) 337059..337334 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
HUZ39_RS01545 (337331) 337331..337690 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HUZ39_RS01550 (337810) 337810..338211 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HUZ39_RS01555 (338217) 338217..339023 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
HUZ39_RS01560 (339020) 339020..339784 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
HUZ39_RS01565 (339784) 339784..340617 - 834 WP_106464827.1 nickel ABC transporter permease subunit NikC -
HUZ39_RS01570 (340614) 340614..341558 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 322030..337690 15660


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T161895 WP_001259388.1 NZ_CP055199:337059-337334 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T161895 NZ_CP071393:c5186322-5186035 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTATCGGTC
TCGTTCATGGGGTCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT161895 WP_000593555.1 NZ_CP055199:337331-337690 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT161895 NZ_CP071393:c5186048-5185746 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References