Detailed information of TA system TA161893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 52426..53027
• Replicon: plasmid unnamed3
• Accession: NZ_CP055197
• Organism: Shigella flexneri strain STEFF_10

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: HUZ40_RS25100
• Protein ID: WP_001216034.1
• Coordinates: 52647..53027 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: HUZ40_RS25095
• Protein ID: WP_001190712.1
• Coordinates: 52426..52647 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ40_RS25065 (HUZ40_24895)	47535..48041	+	507	WP_000021754.1	hypothetical protein	-
HUZ40_RS25070	48236..49414	+	1179	WP_094318253.1	hypothetical protein	-
HUZ40_RS25075 (HUZ40_24910)	49542..49772	+	231	WP_223656858.1	hypothetical protein	-
HUZ40_RS25080 (HUZ40_24915)	50848..51204	-	357	WP_001062545.1	hypothetical protein	-
HUZ40_RS25085	51205..51612	-	408	WP_223656859.1	hypothetical protein	-
HUZ40_RS25090 (HUZ40_24930)	51964..52353	+	390	WP_000506724.1	S24 family peptidase	-
HUZ40_RS25095 (HUZ40_24935)	52426..52647	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HUZ40_RS25100 (HUZ40_24940)	52647..53027	+	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HUZ40_RS25105 (HUZ40_24945)	53032..53211	+	180	WP_000113018.1	hypothetical protein	-
HUZ40_RS25110 (HUZ40_24950)	53239..54282	+	1044	WP_112918711.1	DUF968 domain-containing protein	-
HUZ40_RS25115 (HUZ40_24955)	54371..54823	+	453	WP_001326849.1	late promoter-activating protein	-
HUZ40_RS25120 (HUZ40_24960)	54909..56102	+	1194	WP_032214401.1	hypothetical protein	-
HUZ40_RS25125 (HUZ40_24965)	56144..57586	+	1443	WP_223656860.1	terminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	dfrA14 / tet(A) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib	-	1..78847	78847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T161893 WP_001216034.1 NZ_CP055197:52647-53027 [Shigella flexneri]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T161893 NZ_CP071393:4701585-4701962 [Klebsiella michiganensis]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACAGCACACCCGTCACCTGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACAAGCCGTTTGGCAACGACTGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAACCACCGGGCTGATGACCCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGAAAATATCGAGAGGTGCAGCCATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT161893 WP_001190712.1 NZ_CP055197:52426-52647 [Shigella flexneri]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT161893 NZ_CP071393:4701187-4701534 [Klebsiella michiganensis]
ATTGCTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTTTGGCGCACGGCTGGTGCAGGCGGGCAACCGTCT
GCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACGCGGATTTACGGCATCTCGACCAGGCATTTCCAC
TGCTGCTCAAACAGCTTGAACTGATGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCAATCCCCGCCATCAG
CACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGTATACCTGGCGAT
TTATCCGACACCAGCGGCAACCGCATAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6