Detailed information of TA system TA161887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 6659..7206
• Replicon: plasmid unnamed2
• Accession: NZ_CP055196
• Organism: Shigella flexneri strain STEFF_10

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: HUZ40_RS24285
• Protein ID: WP_244442846.1
• Coordinates: 6928..7206 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: HUZ40_RS24280
• Protein ID: WP_000079941.1
• Coordinates: 6659..6928 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ40_RS24255 (2100)	2100..3308	-	1209	WP_239637886.1	IS91 family transposase	-
HUZ40_RS24260 (3289)	3289..3561	-	273	WP_000019163.1	hypothetical protein	-
HUZ40_RS24265 (3776)	3776..4591	+	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-
HUZ40_RS24270 (4678)	4678..4920	+	243	Protein_4	phosphoglucosamine mutase	-
HUZ40_RS24275 (5156)	5156..6301	-	1146	Protein_5	IS91-like element ISVsa3 family transposase	-
HUZ40_RS24280 (6659)	6659..6928	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
HUZ40_RS24285 (6928)	6928..7206	+	279	WP_244442846.1	type II toxin-antitoxin system toxin YacB	Toxin
HUZ40_RS24290 (7246)	7246..8094	+	849	WP_021265674.1	3'-5' exonuclease	-
HUZ40_RS24295 (8211)	8211..8684	+	474	WP_001334658.1	hypothetical protein	-
HUZ40_RS24300 (9161)	9161..9427	-	267	WP_000955263.1	hypothetical protein	-
HUZ40_RS24305 (9427)	9427..9921	-	495	WP_239637887.1	hypothetical protein	-
HUZ40_RS24310 (9977)	9977..10579	-	603	WP_000517695.1	ProQ/FINO family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2	-	1..99313	99313

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10761.43 Da
• Isoelectric Point: 9.7617

>T161887 WP_244442846.1 NZ_CP055196:6928-7206 [Shigella flexneri]
MEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T161887 NZ_CP071393:c67315-67007 [Klebsiella michiganensis]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTAATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT161887 WP_000079941.1 NZ_CP055196:6659-6928 [Shigella flexneri]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT161887 NZ_CP071393:c67554-67318 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTTAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5