Detailed information of TA system TA161874

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 4163525..4164303
• Replicon: chromosome
• Accession: NZ_CP055194
• Organism: Shigella flexneri strain STEFF_10

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A3U1V6G9
• Locus tag: HUZ40_RS20200
• Protein ID: WP_033805147.1
• Coordinates: 4163525..4163899 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3U1V6I4
• Locus tag: HUZ40_RS20205
• Protein ID: WP_033805146.1
• Coordinates: 4163938..4164303 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ40_RS20195 (4159886)	4159886..4163002	+	3117	WP_000149660.1	HsdR family type I site-specific deoxyribonuclease	-
HUZ40_RS20200 (4163525)	4163525..4163899	-	375	WP_033805147.1	TA system toxin CbtA family protein	Toxin
HUZ40_RS20205 (4163938)	4163938..4164303	-	366	WP_033805146.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUZ40_RS20210 (4164329)	4164329..4164550	-	222	WP_033805145.1	DUF987 domain-containing protein	-
HUZ40_RS20215 (4164547)	4164547..4165089	-	543	WP_033805144.1	DNA repair protein RadC	-
HUZ40_RS20220 (4165102)	4165102..4165542	-	441	WP_033805143.1	antirestriction protein	-
HUZ40_RS20225 (4165573)	4165573..4166394	-	822	WP_047082063.1	DUF932 domain-containing protein	-
HUZ40_RS20230 (4166496)	4166496..4166726	-	231	WP_033805141.1	DUF905 domain-containing protein	-
HUZ40_RS20235 (4166798)	4166798..4167247	-	450	WP_033805140.1	IrmA family protein	-
HUZ40_RS20240 (4167244)	4167244..4167696	-	453	WP_033805139.1	hypothetical protein	-
HUZ40_RS20245 (4167733)	4167733..4168302	-	570	WP_033805138.1	hypothetical protein	-
HUZ40_RS20250 (4168302)	4168302..4169006	-	705	WP_239637851.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	4145233..4174417	29184

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13843.88 Da
• Isoelectric Point: 7.9537

>T161874 WP_033805147.1 NZ_CP055194:c4163899-4163525 [Shigella flexneri]
MQTISSHLTRAAQPCLSPVEIWQLLLTHLLSQHYGLTLNDTPFSDETTIQKHIDAGISLSDAVNFLVEKYELIRIDRDGC
SVMAQKSPFITSIDILRARKASGLMKRSSHKTVTRATAGQHQEL

Nucleotide

• Length: 375 bp

>T161874 NZ_CP071385:350557-350904 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13393.06 Da
• Isoelectric Point: 5.0700

>AT161874 WP_033805146.1 NZ_CP055194:c4164303-4163938 [Shigella flexneri]
MNNHSESGAIPENPPCQQWGLKSTITPCFGARLVQEGDRLHFLADRAGFNGAFSDEHALRLDQAFPLILKQLELMLTSGE
LNPRHPHSVTLYHNGLTCEADTLGSCGYVFIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT161874 NZ_CP071385:350904-351203 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATGGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A3U1V6G9

Antitoxin

• AlphaFold DB: A0A3U1V6I4