Detailed information of TA system TA161872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3581887..3582724
• Replicon: chromosome
• Accession: NZ_CP055194
• Organism: Shigella flexneri strain STEFF_10

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: HUZ40_RS17465
• Protein ID: WP_000227784.1
• Coordinates: 3582182..3582724 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: HUZ40_RS17460
• Protein ID: WP_001297137.1
• Coordinates: 3581887..3582198 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ40_RS17435 (3576907)	3576907..3577854	+	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-
HUZ40_RS17440 (3577876)	3577876..3579867	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HUZ40_RS17445 (3579857)	3579857..3580471	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HUZ40_RS17450 (3580471)	3580471..3580800	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HUZ40_RS17455 (3580812)	3580812..3581702	+	891	WP_000971336.1	heme o synthase	-
HUZ40_RS17460 (3581887)	3581887..3582198	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
HUZ40_RS17465 (3582182)	3582182..3582724	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
HUZ40_RS17470 (3582780)	3582780..3583715	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
HUZ40_RS17475 (3584123)	3584123..3585487	+	1365	WP_001000978.1	MFS transporter	-
HUZ40_RS17480 (3585615)	3585615..3586106	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HUZ40_RS17485 (3586274)	3586274..3587185	+	912	WP_000705849.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T161872 WP_000227784.1 NZ_CP055194:3582182-3582724 [Shigella flexneri]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T161872 NZ_CP071383:c4021138-4020770 [Leclercia sp. 4-9-1-25]
ATGACGCTGCAATTTATCTCAGCGGAAGAGATAGTGCTGTTTCACGACAAGCTGCTTAGCGTGACGCCGGGCGTTGCGGG
AATGCCGGATCCAGGCCGGGCGGAAGCCCTGCTGTATCGCGTGCTGAATAAGTACGAATACGAAGGCGTTACCGATGTCT
GGATGCTGGCGGCGATGCACCTGCTGGCTATCTCTCGCGGTCACATCTTCAATGACGGCAATAAGCGAACGGCTTTGTTT
ATTACCCTGCTGTTTCTCAAACGTAATGGTATTTCGTTAAAGGCCAATCCCGATTATGTGGATATGACCGTTGAGGCAGC
GGCAGGACGTTTGACGCTTGACGAGGTTGTCCAGCGTCTACGTTGTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT161872 WP_001297137.1 NZ_CP055194:3581887-3582198 [Shigella flexneri]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT161872 NZ_CP071383:c4021356-4021135 [Leclercia sp. 4-9-1-25]
ATGCGTACAATGAACTATAGCGAAGCCCGGCAGAATCTTGCCTCCGCGCTCGAGTCCGCGGCAGCAGGAACGCCAGTCAC
TATTTCACGTCGTGGTCATAAGTCTGCAGTGATCATCAGTGTGGAAGAGTTTGAGCGTTATCAGACGGCAAAGCTCGACG
CAGAGTTTGACGCCATCATGGGTATTCATGGCAGCGAAATCAGGGAGCTGGCTGATAAATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3