Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2474897..2475535 Replicon chromosome
Accession NZ_CP055194
Organism Shigella flexneri strain STEFF_10

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HUZ40_RS12035 Protein ID WP_000813794.1
Coordinates 2475359..2475535 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HUZ40_RS12030 Protein ID WP_001270285.1
Coordinates 2474897..2475313 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ40_RS12010 (2470049) 2470049..2470990 - 942 WP_001251315.1 ABC transporter permease -
HUZ40_RS12015 (2470991) 2470991..2472004 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
HUZ40_RS12020 (2472022) 2472022..2473167 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
HUZ40_RS12025 (2473412) 2473412..2474818 - 1407 WP_000760594.1 PLP-dependent aminotransferase family protein -
HUZ40_RS12030 (2474897) 2474897..2475313 - 417 WP_001270285.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HUZ40_RS12035 (2475359) 2475359..2475535 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HUZ40_RS12040 (2475757) 2475757..2475987 + 231 WP_000494244.1 YncJ family protein -
HUZ40_RS12045 (2476079) 2476079..2478040 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HUZ40_RS12050 (2478113) 2478113..2478649 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HUZ40_RS12055 (2478741) 2478741..2479916 + 1176 WP_001236268.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2479956..2481104 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(88-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T161870 WP_000813794.1 NZ_CP055194:c2475535-2475359 [Shigella flexneri]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T161870 NZ_CP071383:c2588221-2588118 [Leclercia sp. 4-9-1-25]
GCAAGGCGACTTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15247.61 Da        Isoelectric Point: 4.6115

>AT161870 WP_001270285.1 NZ_CP055194:c2475313-2474897 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT161870 NZ_CP071383:2588105-2588286 [Leclercia sp. 4-9-1-25]
TTTTCAAGCAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGC
TAAAAGTTGGCATTAATGCAGGCTTAAGTCGCCTTGCACTATAAGAATAGATGACAGCGCCAGGTTTTCCAGTCCGCATC
AAAAGTGGTCAGAAAAAAAGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References