Detailed information of TA system TA161869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2379477..2381065
• Replicon: chromosome
• Accession: NZ_CP055194
• Organism: Shigella flexneri strain STEFF_10

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: F4NFZ0
• Locus tag: HUZ40_RS11640
• Protein ID: WP_001125463.1
• Coordinates: 2379743..2381065 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HUZ40_RS11635
• Protein ID: WP_001296726.1
• Coordinates: 2379477..2379743 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ40_RS11635 (2379477)	2379477..2379743	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HUZ40_RS11640 (2379743)	2379743..2381065	+	1323	WP_001125463.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HUZ40_RS11645 (2381377)	2381377..2381585	+	209	Protein_2281	AraC family transcriptional regulator	-
HUZ40_RS11650 (2382018)	2382018..2382581	+	564	WP_001351339.1	type 1 fimbrial major subunit FimA	-
HUZ40_RS11655 (2382942)	2382942..2383652	+	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2379477..2395565	16088

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49422.05 Da
• Isoelectric Point: 9.1203

>T161869 WP_001125463.1 NZ_CP055194:2379743-2381065 [Shigella flexneri]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T161869 NZ_CP071383:2226299-2226790 [Leclercia sp. 4-9-1-25]
GTGGGAAAAGTAACGGCTCCAGTACCATTGAATTCGTCACATATTCTCGCTGAATTTCACTGTGGTGAAGCTGCACTGGA
CGAATGGATTAAGCACCGGGGGTTAAAAAATCAATCCATGGGAGCTGCAAGGACATTTGTGGTATGCCGGGGCAACGCGT
CTCAGGTGATTGGGTTTTATTCTCTCGCGACCGGTAGCGTCACCCATGCTGTTGCACCGGGAAGCCTGCGCAGGAATATG
CCCGATCCGGTCCCCGTCATCATTCTGGCCCGGCTTGCCGTTGATGCGAGTTTTCACAGCAAAGGACTCGGTGCTGATTT
ACTCCATGACGCCGTCTTACGCTGCTGCCGGGTGGCTGAGAACATTGGCGTACGGGCCATTATGGTTCATGCTATTTCCG
ACCGCGCCAGGCAGTTTTACATCCATCACGGATTCACCCCTTCGCAAACGCAGGAGAGAACCTTATTCCTGCGATTGCCG
ATCATAACTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT161869 WP_001296726.1 NZ_CP055194:2379477-2379743 [Shigella flexneri]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT161869 NZ_CP071383:2226024-2226311 [Leclercia sp. 4-9-1-25]
TTGCTATACAAAGGAGGAAGTATGAAATCTGATGTTCAGCTTAATATCCGGGCAAAAGAAGCGCAGCGAGCCCTGATTGA
TGCTGCCGCTGAGATTCTCCATAAATCACGCACCGATTTTATCCTGGAGATGGCCTGCCAGGCAGCTGAGAACGTCATCC
TTGACCGCCGGATGTTTAATCTTAACGATGAACAATATGCCGAATTTATCGAGATGCTGGATGCCCCGGTGACTGCTAAT
CCGGCGATGGATAAACTCCTGGCGAGAAAACCGCAGTGGGAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9IYX0