Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1323792..1324417 Replicon chromosome
Accession NZ_CP055194
Organism Shigella flexneri strain STEFF_10

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag HUZ40_RS06445 Protein ID WP_000911329.1
Coordinates 1324019..1324417 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HUZ40_RS06440 Protein ID WP_000450524.1
Coordinates 1323792..1324019 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ40_RS06415 (1319594) 1319594..1320064 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
HUZ40_RS06420 (1320064) 1320064..1320636 - 573 WP_000176191.1 glycine cleavage system transcriptional repressor -
HUZ40_RS06425 (1320782) 1320782..1321660 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HUZ40_RS06430 (1321677) 1321677..1322711 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HUZ40_RS06435 (1322924) 1322924..1323637 + 714 WP_154388146.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HUZ40_RS06440 (1323792) 1323792..1324019 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HUZ40_RS06445 (1324019) 1324019..1324417 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ40_RS06450 (1324564) 1324564..1325427 + 864 WP_001267498.1 neutral zinc metallopeptidase -
HUZ40_RS06455 (1325442) 1325442..1327457 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
HUZ40_RS06460 (1327531) 1327531..1328229 + 699 WP_000679823.1 esterase -
HUZ40_RS06465 (1328339) 1328339..1328539 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T161858 WP_000911329.1 NZ_CP055194:1324019-1324417 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T161858 NZ_CP071382:618656-618958 [Geobacter sp. Jerry-YX]
ATGCCTTCTTTCACCCTCACCAACCTGGCGAAAGCTGATTTGAAAGAAATCGGACAGTACACACAAAAGCATTGGGGGCG
CGACCAACGCAACCTCTATCTGGGAATGCCGGATGCTTCCTTTCGGCAACTGGCAGACAATCTCCTTGCGGGTAAGGATT
GCAGCGATATTCGGGATGGGTACCGGAAATTCCGCACCGGTAGCCATGTAATCTTTTATCGGCAAAAGTCCGATGACGCC
ATCGAGATTGTCCGTATTCTGCATGGGCGCATGGATTGTGACACGCGGCTTTATTCGGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT161858 WP_000450524.1 NZ_CP055194:1323792-1324019 [Shigella flexneri]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT161858 NZ_CP071382:618414-618656 [Geobacter sp. Jerry-YX]
ATGCAAAAAAATACCAGTGTCACGTTAGGTCCGCACTACGAGAAATTCATTTCCCAGCAGGTCGCCCAGGGCCGCTTTGG
TTCGGCAAGCGAGGCTATCCGGGCTGGTCTACGGCTTCTGGAGGAACGGGAGACCAAGCTTTCCCTGTTGCGACGCGCGC
TTATCGAAGGTGATGAAAGCGGCACCGCCGATTATTCCCTGAAGGACCTGATCAATGAGATTGACGATGAAGGTCTCAAC
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References