Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 858319..858973 Replicon chromosome
Accession NZ_CP055194
Organism Shigella flexneri strain STEFF_10

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HUZ40_RS04190 Protein ID WP_000244781.1
Coordinates 858566..858973 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ40_RS04185 Protein ID WP_000354046.1
Coordinates 858319..858585 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ40_RS04160 (853488) 853488..854231 + 744 WP_000951951.1 SDR family oxidoreductase -
HUZ40_RS04165 (854288) 854288..855721 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
HUZ40_RS04170 (855766) 855766..856077 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HUZ40_RS04175 (856241) 856241..856900 + 660 WP_000250274.1 hemolysin III family protein -
HUZ40_RS04180 (857096) 857096..858076 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
HUZ40_RS04185 (858319) 858319..858585 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ40_RS04190 (858566) 858566..858973 + 408 WP_000244781.1 protein YgfX Toxin
HUZ40_RS04195 (859013) 859013..859534 - 522 WP_001055874.1 flavodoxin FldB -
HUZ40_RS04200 (859646) 859646..860542 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ40_RS04205 (860567) 860567..861277 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ40_RS04210 (861283) 861283..863016 + 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T161856 WP_000244781.1 NZ_CP055194:858566-858973 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T161856 NZ_CP071382:208059-208340 [Geobacter sp. Jerry-YX]
ATGATTACATCGTTCGCGAACAAGGACTCGGAAAAACTCTTTGCGACGGGGAAATCCAGGAAATTCCCTCCGGAAATCAT
TACTCGCGCGATTATGCGACTAACCCAGTTGGACAGCGCACGCGATGTTACAGATTTGTTGATGCCGCCGTCAAACAGGC
TTGAATCTTTATCCGGCAACAGGTCAGGTCAGTGGAGCATCAGGATTAATGACCAGTGGCGGATATGTTTTTCATTCGCC
AACGGCGAAGCAACTGAGGTTGAAATAACTGACTATCATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT161856 WP_000354046.1 NZ_CP055194:858319-858585 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT161856 NZ_CP071382:208352-208654 [Geobacter sp. Jerry-YX]
ATGGCGACAAAAAATGGCCTTCCTCCTATACATCCCGGAGTGTTCTTGAAAGAAATTCTCGATGAGCTTGGCATCTCGCA
GAACGCTTTTGCGCAAGCAATCGGCGTGTCGCCGATGCGAGTTTCGCATGTAATCAAGGGCACACGTCCTGTCACCGCAG
AACTGGCACTCCTATTCGGAAAGGCCTTCGGCCAGACCCCGATCTACTGGATGAACCTGCAAACCAGCTATGACCTGAAG
ACGGTGGAAAAAGAGCTGGCTCCGAAGGTTCGACAAGTGCACCCGCTTTCGCGGGCAGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References