Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 35195..35838 Replicon plasmid unnamed2
Accession NZ_CP055188
Organism Klebsiella pneumoniae strain STEFF_14

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag HUZ42_RS26480 Protein ID WP_000754566.1
Coordinates 35422..35838 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HUZ42_RS26475 Protein ID WP_001261276.1
Coordinates 35195..35425 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ42_RS26440 (HUZ42_26395) 30314..30583 + 270 WP_000339857.1 hypothetical protein -
HUZ42_RS26445 (HUZ42_26400) 30760..31626 - 867 WP_004118283.1 replication initiation protein -
HUZ42_RS26450 (HUZ42_26405) 32156..32260 + 105 WP_032409716.1 hypothetical protein -
HUZ42_RS26455 (HUZ42_26410) 32389..32646 + 258 WP_000764642.1 hypothetical protein -
HUZ42_RS26460 (HUZ42_26415) 32704..33480 - 777 WP_000015958.1 site-specific integrase -
HUZ42_RS26465 (HUZ42_26420) 33477..34220 - 744 WP_000129823.1 hypothetical protein -
HUZ42_RS26470 (HUZ42_26425) 34271..34621 - 351 WP_000493378.1 hypothetical protein -
HUZ42_RS26475 (HUZ42_26430) 35195..35425 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ42_RS26480 (HUZ42_26435) 35422..35838 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ42_RS26485 (HUZ42_26440) 36042..36898 + 857 Protein_39 IS3-like element ISEc15 family transposase -
HUZ42_RS26490 (HUZ42_26445) 37180..38256 + 1077 WP_000227969.1 IS110 family transposase -
HUZ42_RS26495 (HUZ42_26450) 38273..38581 + 309 Protein_41 integrase core domain-containing protein -
HUZ42_RS26500 (HUZ42_26455) 38556..38906 - 351 Protein_42 tyrosine-type recombinase/integrase -
HUZ42_RS26505 (HUZ42_26460) 38969..39673 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
HUZ42_RS26510 39564..39971 - 408 WP_074428709.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(3')-Ia / aac(3)-IId / blaTEM-1B / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrB52 - 1..58759 58759


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T161853 WP_000754566.1 NZ_CP055188:35422-35838 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T161853 NZ_CP071375:c4642275-4641748 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT161853 WP_001261276.1 NZ_CP055188:35195-35425 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT161853 NZ_CP071375:c4642548-4642282 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAACATCGGCGGG
TGATCCTCAATGAGGAATCCTGGATGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References