Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5232327..5232952 Replicon chromosome
Accession NZ_CP055186
Organism Klebsiella pneumoniae strain STEFF_14

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ42_RS25310 Protein ID WP_002882817.1
Coordinates 5232327..5232710 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ42_RS25315 Protein ID WP_004150355.1
Coordinates 5232710..5232952 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ42_RS25295 (5229693) 5229693..5230595 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ42_RS25300 (5230592) 5230592..5231227 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ42_RS25305 (5231224) 5231224..5232153 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ42_RS25310 (5232327) 5232327..5232710 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ42_RS25315 (5232710) 5232710..5232952 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ42_RS25320 (5233157) 5233157..5234074 + 918 WP_004181612.1 alpha/beta hydrolase -
HUZ42_RS25325 (5234088) 5234088..5235029 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ42_RS25330 (5235074) 5235074..5235511 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ42_RS25335 (5235508) 5235508..5236368 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ42_RS25340 (5236362) 5236362..5236961 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T161852 WP_002882817.1 NZ_CP055186:c5232710-5232327 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T161852 NZ_CP071375:4595972-4596457 [Escherichia coli]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCTCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT161852 WP_004150355.1 NZ_CP055186:c5232952-5232710 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT161852 NZ_CP071375:4595703-4595981 [Escherichia coli]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCGTTGATGACGAGCAATACGATGCGTTTCTGGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCATTGATGAATCGTAAATCGCCATGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References