Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4018484..4019103 Replicon chromosome
Accession NZ_CP055186
Organism Klebsiella pneumoniae strain STEFF_14

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUZ42_RS19635 Protein ID WP_002892050.1
Coordinates 4018885..4019103 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUZ42_RS19630 Protein ID WP_002892066.1
Coordinates 4018484..4018858 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ42_RS19620 (4013636) 4013636..4014829 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ42_RS19625 (4014852) 4014852..4017998 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HUZ42_RS19630 (4018484) 4018484..4018858 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUZ42_RS19635 (4018885) 4018885..4019103 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HUZ42_RS19640 (4019262) 4019262..4019828 + 567 WP_002892030.1 maltose O-acetyltransferase -
HUZ42_RS19645 (4019800) 4019800..4019940 - 141 WP_004147370.1 hypothetical protein -
HUZ42_RS19650 (4019961) 4019961..4020431 + 471 WP_002892026.1 YlaC family protein -
HUZ42_RS19655 (4020406) 4020406..4021857 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HUZ42_RS19660 (4021958) 4021958..4022656 + 699 WP_002892021.1 GNAT family protein -
HUZ42_RS19665 (4022653) 4022653..4022793 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HUZ42_RS19670 (4022793) 4022793..4023056 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161848 WP_002892050.1 NZ_CP055186:4018885-4019103 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T161848 NZ_CP071375:c3887676-3887365 [Escherichia coli]
ATGCACATCATTTCTAAAGTGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT161848 WP_002892066.1 NZ_CP055186:4018484-4018858 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT161848 NZ_CP071375:c3887368-3886949 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTTAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References