Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 661818..662497 Replicon chromosome
Accession NZ_CP055186
Organism Klebsiella pneumoniae strain STEFF_14

Toxin (Protein)


Gene name ykfI Uniprot ID A0A0C7K7A4
Locus tag HUZ42_RS03250 Protein ID WP_020324801.1
Coordinates 662156..662497 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0C7KEL2
Locus tag HUZ42_RS03245 Protein ID WP_020324792.1
Coordinates 661818..662135 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ42_RS03220 (657032) 657032..660184 + 3153 WP_022631414.1 AIDA repeat-containing protein -
HUZ42_RS03225 (660277) 660277..660516 + 240 WP_020324820.1 DUF905 domain-containing protein -
HUZ42_RS03230 (660619) 660619..661077 + 459 WP_020324813.1 antirestriction protein -
HUZ42_RS03235 (661093) 661093..661569 + 477 WP_020324797.1 RadC family protein -
HUZ42_RS03240 (661578) 661578..661805 + 228 WP_020324796.1 DUF987 domain-containing protein -
HUZ42_RS03245 (661818) 661818..662135 + 318 WP_020324792.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HUZ42_RS03250 (662156) 662156..662497 + 342 WP_020324801.1 TA system toxin CbtA family protein Toxin
HUZ42_RS03255 (662613) 662613..663446 + 834 WP_020324805.1 DUF4942 domain-containing protein -
HUZ42_RS03265 (663749) 663749..664255 + 507 WP_002917636.1 G/U mismatch-specific DNA glycosylase -
HUZ42_RS03270 (664355) 664355..666196 - 1842 WP_004174339.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 644079..663446 19367


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-111)

Antitoxin

Antitoxin CbeA

(5-103)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12818.91 Da        Isoelectric Point: 9.1484

>T161836 WP_020324801.1 NZ_CP055186:662156-662497 [Klebsiella pneumoniae]
MKTLPAIISRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFCDETVIQEHIDAGITLADAVNFLVEKYGLVRIDRNGF
VWQEQSPYLRAVDILRARQATGLLRQSRNNAIR

Download         Length: 342 bp

>T161836 NZ_CP071375:803080-803394 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11915.51 Da        Isoelectric Point: 6.6246

>AT161836 WP_020324792.1 NZ_CP055186:661818-662135 [Klebsiella pneumoniae]
MSNITWGLQRDTTPRLGARLVQEGNQLHYLADRASMTGKFSDAECRKLDETFPHFISQMESMLITGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAVYPIQR

Download         Length: 318 bp

>AT161836 NZ_CP071375:802844-803077 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTAGCTGATGAAACCGGATGTTTCTCTGAGGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7K7A4

Antitoxin

Source ID Structure
AlphaFold DB A0A0C7KEL2

References