Detailed information of TA system TA161829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3550877..3551714
• Replicon: chromosome
• Accession: NZ_CP055184
• Organism: Shigella flexneri strain STEFF_16

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: HUZ44_RS17170
• Protein ID: WP_000227784.1
• Coordinates: 3551172..3551714 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: HUZ44_RS17165
• Protein ID: WP_001297137.1
• Coordinates: 3550877..3551188 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ44_RS17140 (3545897)	3545897..3546844	+	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-
HUZ44_RS17145 (3546866)	3546866..3548857	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HUZ44_RS17150 (3548847)	3548847..3549461	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HUZ44_RS17155 (3549461)	3549461..3549790	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HUZ44_RS17160 (3549802)	3549802..3550692	+	891	WP_000971336.1	heme o synthase	-
HUZ44_RS17165 (3550877)	3550877..3551188	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
HUZ44_RS17170 (3551172)	3551172..3551714	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
HUZ44_RS17175 (3551770)	3551770..3552705	-	936	WP_032197347.1	tetratricopeptide repeat protein	-
HUZ44_RS17180 (3553113)	3553113..3554477	+	1365	WP_001000978.1	MFS transporter	-
HUZ44_RS17185 (3554605)	3554605..3555096	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HUZ44_RS17190 (3555264)	3555264..3556175	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T161829 WP_000227784.1 NZ_CP055184:3551172-3551714 [Shigella flexneri]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T161829 NZ_CP071324:c2716257-2715772 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAACCAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT161829 WP_001297137.1 NZ_CP055184:3550877-3551188 [Shigella flexneri]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT161829 NZ_CP071324:c2716511-2716245 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAGCTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3