Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3516798..3517416 Replicon chromosome
Accession NZ_CP055184
Organism Shigella flexneri strain STEFF_16

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ44_RS17000 Protein ID WP_001291435.1
Coordinates 3517198..3517416 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ44_RS16995 Protein ID WP_000344800.1
Coordinates 3516798..3517172 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ44_RS16985 (3511887) 3511887..3513080 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ44_RS16990 (3513103) 3513103..3516252 + 3150 WP_001132462.1 efflux RND transporter permease AcrB -
HUZ44_RS16995 (3516798) 3516798..3517172 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ44_RS17000 (3517198) 3517198..3517416 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ44_RS17005 (3517588) 3517588..3518139 + 552 WP_000102568.1 maltose O-acetyltransferase -
HUZ44_RS17010 (3518255) 3518255..3518725 + 471 WP_000136192.1 YlaC family protein -
HUZ44_RS17015 (3518889) 3518889..3520439 + 1551 WP_001364591.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ44_RS17020 (3520481) 3520481..3520834 - 354 WP_000878141.1 DUF1428 family protein -
HUZ44_RS17030 (3521213) 3521213..3521524 + 312 WP_000409911.1 MGMT family protein -
HUZ44_RS17035 (3521555) 3521555..3522127 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161828 WP_001291435.1 NZ_CP055184:3517198-3517416 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161828 NZ_CP071324:c2583929-2583561 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCATGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTATGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161828 WP_000344800.1 NZ_CP055184:3516798-3517172 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161828 NZ_CP071324:c2584147-2583926 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References