Detailed information of TA system TA161818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1318003..1318628
• Replicon: chromosome
• Accession: NZ_CP055184
• Organism: Shigella flexneri strain STEFF_16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUZ44_RS06335
• Protein ID: WP_000911330.1
• Coordinates: 1318230..1318628 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HUZ44_RS06330
• Protein ID: WP_000450524.1
• Coordinates: 1318003..1318230 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ44_RS06305 (1313806)	1313806..1314276	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HUZ44_RS06310 (1314276)	1314276..1314848	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HUZ44_RS06315 (1314994)	1314994..1315872	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HUZ44_RS06320 (1315889)	1315889..1316923	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
HUZ44_RS06325 (1317136)	1317136..1317849	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HUZ44_RS06330 (1318003)	1318003..1318230	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HUZ44_RS06335 (1318230)	1318230..1318628	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ44_RS06340 (1318775)	1318775..1319638	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
HUZ44_RS06345 (1319653)	1319653..1321668	+	2016	WP_032197281.1	tRNA cytosine(34) acetyltransferase TmcA	-
HUZ44_RS06350 (1321742)	1321742..1322440	+	699	WP_000679823.1	esterase	-
HUZ44_RS06355 (1322550)	1322550..1322750	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T161818 WP_000911330.1 NZ_CP055184:1318230-1318628 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T161818 NZ_CP071320:3931120-3931323 [Serratia ureilytica]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAAAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCCGCCGATCATCGTTTGGCGGAGCTGACCATGAACAAGC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT161818 WP_000450524.1 NZ_CP055184:1318003-1318230 [Shigella flexneri]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT161818 NZ_CP071320:3930701-3931069 [Serratia ureilytica]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAACTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
CACCCTGGGAGACAGCCACCACGGGTGGGTCAACGATCCGACATCCTCCGTCAACCTTCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCCGATTTGTCGGAGTTAGTCGAGGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTACGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33