Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1011092..1011675 Replicon chromosome
Accession NZ_CP055184
Organism Shigella flexneri strain STEFF_16

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag HUZ44_RS04840 Protein ID WP_000254739.1
Coordinates 1011340..1011675 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HUZ44_RS04835 Protein ID WP_000581937.1
Coordinates 1011092..1011340 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ44_RS04825 (1007431) 1007431..1008732 + 1302 WP_000046789.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
HUZ44_RS04830 (1008780) 1008780..1011014 + 2235 WP_000226815.1 GTP pyrophosphokinase -
HUZ44_RS04835 (1011092) 1011092..1011340 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HUZ44_RS04840 (1011340) 1011340..1011675 + 336 WP_000254739.1 endoribonuclease MazF Toxin
HUZ44_RS04845 (1011746) 1011746..1012537 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HUZ44_RS04850 (1012765) 1012765..1014402 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HUZ44_RS04855 (1014490) 1014490..1015788 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12126.09 Da        Isoelectric Point: 8.2618

>T161817 WP_000254739.1 NZ_CP055184:1011340-1011675 [Shigella flexneri]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVVPEELQLIKAKINVLIG

Download         Length: 336 bp

>T161817 NZ_CP071320:3299014-3299298 [Serratia ureilytica]
ATGACTTATAAGCTTGAATTCGAAGAACATGCGCTGAAGGAATTTAAAAAACTGTCCCCGGTGATCCGGGAACAGTTCAA
AAAAAAGCTGGCATCGGTGCTGGTTAATCCCCACGTCCCCGCCAACAAACTCGCCGGTTTGCCCGATTGCTACAAAATCA
AACTCAGAGCCTCAGGCTTTCGTTTGGTATATCGCGTCATGGAAACGGAGATCGTGGTTCTGGTCCTGTCTGTGGGCAAA
CGCGAACGTTCAGCGGCTTACACCGCTGCCAAAAAGCGCCTTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT161817 WP_000581937.1 NZ_CP055184:1011092-1011340 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT161817 NZ_CP071320:3298773-3299024 [Serratia ureilytica]
ATGACGGTTCAACCCATTCTCGCCGACTCCGCAGTCAGCATCAGCGATTTTAAAAAATCGCCCAACGCCGCCCTTAAAGA
GGCACAAGGGCAACCGGTCGCCGTCCTGACCAACGGTAAGATTTCGGGATATTACGTCTCTCCGGAAACCTGGGAGGCGA
TCGCGGAATACCTGGAAGATATCGAACTCGCTGAAATCGTTCGTTCACGGATGAACGGCAAACGCATTAAGGTCAATTTG
GATGACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References