Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4148241..4148738 Replicon chromosome
Accession NZ_CP055181
Organism Shigella flexneri strain STEFF_17

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUZ45_RS20125 Protein ID WP_001173343.1
Coordinates 4148241..4148414 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A3W4RHQ7
Locus tag HUZ45_RS20130 Protein ID WP_001600874.1
Coordinates 4148442..4148738 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ45_RS20115 (4144911) 4144911..4146368 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HUZ45_RS20120 (4146605) 4146605..4148122 + 1518 WP_001295090.1 lysine--tRNA ligase -
HUZ45_RS20125 (4148241) 4148241..4148414 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUZ45_RS20130 (4148442) 4148442..4148738 - 297 WP_001600874.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUZ45_RS20135 (4148965) 4148965..4149237 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
HUZ45_RS20140 (4149249) 4149249..4149479 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
HUZ45_RS20145 (4149660) 4149660..4151291 + 1632 WP_001216477.1 sensor histidine kinase -
HUZ45_RS20150 (4151288) 4151288..4152007 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4139387..4148738 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T161813 WP_001173343.1 NZ_CP055181:c4148414-4148241 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T161813 NZ_CP071320:842972-843394 [Serratia ureilytica]
GTGGCCCAGTGGCGATGTGATATCCGCATTTCCTGGCGCACCCAGCTGTTCTCTCTGTTGACCCACGGCGTCCTGATCCT
GCTGATCCTGATTTCGCCCTGGCCGGAAGGCTTTGGTCCGCTCTGGCTGGTGTTGCTGACGCTGGTGGTGTTTCAGTGCA
TTCGCAGCCAGAAGCGCATCGCTGCGGTGCAGGGTGAACTGCGGCTGCTGGCGGACAGGCGTTTCAGCTGGCACGGCCGA
GAATGGCAGCTGGCGAAAAAGCCCTGGATGCCGGGCTACGGCCTGCTGCTGACGCTGCAGCCGACGGAGGGCAAAAAGCG
CCGCCGGCTGTGGCTGGCTTCCGACTGCATGAGCAAAGAGGAGTGGCGCCACCTGCGGCAGCTGTTGCTGTATCCGCCGG
CGGGTGACGGCGAGGAGGCCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11482.00 Da        Isoelectric Point: 4.1705

>AT161813 WP_001600874.1 NZ_CP055181:c4148738-4148442 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRTLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT161813 NZ_CP071320:842725-842991 [Serratia ureilytica]
ATGGATATTAACAACAAAGCACGTATTCACTGGGCTTGCCGTCGTGGCATGCGCGAGCTGGACATCTCCATCATGCCGTT
CTTCGAATACGAATACGACAGCCTGAATGACGCGGACAAGGCGCTGTTCATCCGTCTGCTCGAGTGCGACGATCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCGCCGCAGGACAGCGAGCTGCAAAGAATGGTGACCCTGATCCAAACGCGAAAT
AAAGATCGTGGCCCAGTGGCGATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A3W4RHQ7

References