Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3447164..3447782 Replicon chromosome
Accession NZ_CP055181
Organism Shigella flexneri strain STEFF_17

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ45_RS16760 Protein ID WP_001291435.1
Coordinates 3447564..3447782 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ45_RS16755 Protein ID WP_000344800.1
Coordinates 3447164..3447538 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ45_RS16745 (3442253) 3442253..3443446 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ45_RS16750 (3443469) 3443469..3446618 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ45_RS16755 (3447164) 3447164..3447538 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ45_RS16760 (3447564) 3447564..3447782 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ45_RS16765 (3447954) 3447954..3448505 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ45_RS16770 (3448621) 3448621..3449091 + 471 WP_000136192.1 YlaC family protein -
HUZ45_RS16775 (3449255) 3449255..3450805 + 1551 WP_063501968.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ45_RS16780 (3450847) 3450847..3451200 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HUZ45_RS16790 (3451579) 3451579..3451890 + 312 WP_000409911.1 MGMT family protein -
HUZ45_RS16795 (3451921) 3451921..3452493 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161811 WP_001291435.1 NZ_CP055181:3447564-3447782 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161811 NZ_CP071320:c298392-298042 [Serratia ureilytica]
ATGGTGAAGCGCCCCGTTTTTCAGCGCGGAGATCTTATCCGGGTCAGCCTGAATCCCGTGGTTGGCCGTGAGCAGCAAGG
CGACTTTCGTCCGGCACTGGTGCTTTCTCCCCGCGAGTTTAATGCGCTGGGCATGGTGCTAGTTGCCCCTATTTCACAAG
GGGCTAATTTTGCCCGTACAGCGGGTTTCACCGTCAGCCTGAGCGGGAGCGGCACCGCAACGCAGGGCGTCATCTTGATC
AACCAGGTTCGCATGATGGATCTGGCCGGGCGCAGCGCGCAATTCGTTGAACGTGCTCCTGGCGAAGTCATCGCCGACGC
GCTGGCACGGCTTCAGGCAATCATCGACTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161811 WP_000344800.1 NZ_CP055181:3447164-3447538 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161811 NZ_CP071320:c298634-298386 [Serratia ureilytica]
ATGAAAGTCTTTATCAAAAAATGGGGCAACAGCGCAGGAATGCTGGTGCCCTCAGCGCTAATGAACGAGCTGGAACTCCA
GGTAGGTCAGGAAATGGACATGCAGGTCATCGATGGAACGTTGGTCATTAAACCGGTTAAGAAACGCTATTCACTGCAGC
AATTGCTCGATAGCTGTGACGGTGAAGCGCCGGCCGCCGCAGACGACAACTGGGAACACATGCCGCCGGCGGGGAAAGAA
GTATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References